This study investigated the effects of Lactobacillus curvatus fermentation on the oxidative stress attenuating effects of Euryale ferox on H₂O₂-induced human skin fibroblasts (HSF). The results showed that Lactobacillus curvatus fermentation (i) increases the content of the various bioactive components of Euryale ferox and is found to have smaller molecular weights of polysaccharides and polypeptides; (ii) increases the overall intracellular and extracellular antioxidant capacity of H₂O₂-induced HSF while reducing reactive oxygen species (ROS) levels. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) all showed simultaneous increases in activity. Aside from that, the Nrf2 and MAPK signaling pathways are activated to regulate downstream-associated proteins such as the Bax/Bcl-2 protein ratio, matrix metalloproteinase 1 (MMP-1) activity, and human type I collagen (COL-1). These results suggested that the fermentation of Euryale ferox with Lactobacillus curvatus enhances its antioxidant capacity and attenuates apoptosis and senescence caused by oxidative stress.

中文翻译：

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用弯曲乳杆菌增强 Euryale ferox 的生物活性成分以减少 H2O2 诱导的人体皮肤成纤维细胞氧化应激

本研究调查了弯曲乳杆菌发酵对Euryale ferox对 H ₂ O ₂诱导的人类皮肤成纤维细胞 (HSF) 的氧化应激减弱作用的影响。结果表明，曲氏乳杆菌发酵 (i) 增加了Euryale ferox各种生物活性成分的含量，并发现具有较小的多糖和多肽分子量；(ii) 增加 H ₂ O _{2的整体细胞内和细胞外抗氧化能力}- 诱导 HSF，同时降低活性氧 (ROS) 水平。超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-Px) 和过氧化氢酶 (CAT) 均表现出同时增加的活性。除此之外，Nrf2 和 MAPK 信号通路被激活以调节下游相关蛋白，例如 Bax/Bcl-2 蛋白比率、基质金属蛋白酶 1 (MMP-1) 活性和人 I 型胶原蛋白 (COL-1)。这些结果表明，用弯曲乳杆菌发酵Euryale ferox可增强其抗氧化能力，并减轻由氧化应激引起的细胞凋亡和衰老。