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The Temporal Topography of Central Serous Chorioretinopathy in the Chinchilla Rabbits Induced by Intravenous Injection of Adrenaline: An in vivo Study
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2022-09-23 , DOI: 10.2147/dddt.s381957
Weiming Yan 1, 2 , Pan Long 3 , Lei Zhang 4 , Meizhu Chen 1 , Zuoming Zhang 2 , Tao Chen 2
Affiliation  

Purpose: To explore the temporal topography of the chorioretinopathy in an animal model of central serous chorioretinopathy (CSC) induced by intravenous injection of adrenalin in the Chinchilla rabbits.
Methods: Ten Chinchilla rabbits received a daily intravenous injection of adrenaline at 0.04 mg/kg for 8 weeks. Fluorescence fundus angiography (FFA) and electroretinogram (ERG) were performed every week afterwards to see whether there was fluorescence leakage in the fundus and to evaluate the retinal function. Indocyanine green angiography (ICGA) and optical coherence tomography (OCT) were also conducted to detect the change of choroidal vessels. Finally, the eyes of the rabbits were enucleated to make the retinal sections for histological examination with hematoxylin–eosin (HE) staining.
Results: Within 8 weeks of the adrenalin administration, 7 out of the 10 rabbits showed different degrees of fluorescence leakage on FFA. The leakage was more obvious during 2– 3 weeks after the adrenalin administration. With the progress of disease, the leakage subsided gradually and a scar-like lesion formed. ICGA revealed the local choroidal ischemia and the dilated choroidal vessels. An obvious detachment of retina and an increased thickness of the choroid were found on OCT, which was most obvious 2 weeks after the adrenalin administration (P< 0.01). ERG revealed no obvious decline of the b-wave amplitude before and after the adrenalin administration (P> 0.05). A circumscribed retinal detachment, the depigmentation of retinal pigment epithelium and enlarged choroidal vessels were shown by the histological examination.
Conclusion: The temporal topography of the chorioretinopathy in the Chinchilla rabbits by intravenous injection of adrenaline somewhat mimicked that of the human CSC, which could enhance its application in the exploration for the pathogenesis and the therapeutic measures for human CSC.

Keywords: central serous chorioretinopathy, adrenaline, Chinchilla rabbits, choroid


中文翻译:

静脉注射肾上腺素引起的龙猫兔中央浆液性脉络膜视网膜病变的时间分布:一项体内研究

目的:探讨静脉注射肾上腺素诱导的栗鼠兔中心性浆液性脉络膜视网膜病变(CSC)动物模型中脉络膜视网膜病变的时间形貌。
方法: 10 只栗鼠兔每天静脉注射 0.04 mg/kg 肾上腺素,持续 8 周。之后每周进行荧光眼底血管造影(FFA)和视网膜电图(ERG),观察眼底是否有荧光渗漏,评估视网膜功能。还进行了吲哚菁绿血管造影(ICGA)和光学相干断层扫描(OCT)以检测脉络膜血管的变化。最后,摘除兔子的眼睛,制作视网膜切片,用苏木精-伊红(HE)染色进行组织学检查。
结果:肾上腺素给药后8周内,10只兔子中有7只在FFA上表现出不同程度的荧光渗漏。在肾上腺素给药后 2-3 周内渗漏更为明显。随着病情的发展,渗漏逐渐消退,形成瘢痕样病变。ICGA 显示局部脉络膜缺血和扩张的脉络膜血管。OCT可见视网膜明显脱离,脉络膜增厚,肾上腺素给药后2周最为明显(P <0.01)。ERG 显示肾上腺素给药前后 b 波幅值无明显下降(P> 0.05)。组织学检查显示局限性视网膜脱离、视网膜色素上皮色素脱失和脉络膜血管扩大。
结论:静脉注射肾上腺素对栗鼠兔脉络膜视网膜病变的时间形貌与人CSC有一定的相似性,可增强其在探索人CSC发病机制和治疗措施中的应用。

关键词:中央浆液性脉络膜视网膜病变,肾上腺素,栗鼠兔,脉络膜
更新日期:2022-09-23
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