In the present study we report the efficacy of food matrices derived from olives in preventing oxidation of low-density lipoprotein cholesterol (LDL) and lipid peroxidation. To this end, 12 healthy volunteers were divided into 3 groups and cross-received a single dose of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), or as fortified extra virgin olive oil (12.19 mg HT + tyrosol). Blood and urine samples were collected at baseline and up to 12 hours after ingestion. Plasma oxidized LDL levels were measured by ELISA using a monoclonal antibody, while F2-isoprostanes were quantified in urine by UHPLC-MS/MS. Despite the great variability between individuals, a tendency to reduce lipoxidation reactions has been observed after olive phenolics intake in both, blood and urine. In addition, the subgroup of individuals with the highest baseline lipoxidation level showed a decrease in F2-isoprostanes (p < 0.05) after taking the food supplements, as well as a marked decrease in oxidized LDL levels (p < 0.01) after intake of the food supplement with the lowest HT dose. These promising results suggest that HT supplementation could be a useful aid in preventing lipoxidation. Additionally, people with a redox imbalance could benefit even more from supplementing with bioavailable HT.

中文翻译：

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富含羟基酪醇的食品补充剂对预防健康男性脂质过氧化的生物功效

在本研究中，我们报告了源自橄榄的食物基质在防止低密度脂蛋白胆固醇 (LDL) 氧化和脂质过氧化方面的功效。为此，将 12 名健康志愿者分成 3 组并交叉接受单剂量的橄榄酚类物质，主要是羟基酪醇 (HT)，以液体膳食补充剂 (30.6 或 61.5 mg HT) 或强化特级初榨橄榄油的形式提供(12.19 毫克 HT + 酪醇)。在基线和摄入后长达 12 小时收集血液和尿液样本。使用单克隆抗体通过 ELISA 测量血浆氧化的 LDL 水平，而通过 UHPLC-MS/MS 对尿液中的 F2-异前列腺素进行定量。尽管个体之间存在很大差异，但在血液和尿液中摄入橄榄酚后，已经观察到减少脂氧化反应的趋势。此外，具有最高基线脂氧化水平的个体亚组在服用食品补充剂后显示 F2-异前列腺素下降 (p < 0.05)，并且在摄入含HT 剂量最低。这些有希望的结果表明，HT 补充剂可能有助于预防脂肪氧化。此外，氧化还原失衡的人可以从补充生物可利用的 HT 中受益更多。这些有希望的结果表明，HT 补充剂可能有助于预防脂肪氧化。此外，氧化还原失衡的人可以从补充生物可利用的 HT 中受益更多。这些有希望的结果表明，HT 补充剂可能有助于预防脂肪氧化。此外，氧化还原失衡的人可以从补充生物可利用的 HT 中受益更多。