Chlorpyrifos (CPF), as an extensively used organophosphorus pesticide, often remains on food surfaces or contaminates water sources. CPF can cause many toxic effects on human production and life. As an additional product of non-medicinal parts of ginseng, the pharmacological activity of ginseng stem and leaf total saponin (GSLS) has been verified and applied in recent years. This study aimed to evaluate the protective effect of GSLS on CPF-induced liver damage in mice. Experimental results in vivo demonstrate that GSLS can reduce the accumulation of oxidation product MDA by relieving CPF-induced liver function indicators in mice and enhancing the antioxidant enzyme SOD and CAT activities of mice. With the decrease in mRNA expression of BAX, NF-KB, and TIMP in liver tissues, the mRNA expression of Nrf-2, HO-1, and XIAP increased. GSLS can alleviate CPF-induced liver toxicity through anti-inflammatory, antioxidant, and anti-inflammatory. In vitro experiments have proved that GSLS can show the ability to scavenge DPPH free radicals and hydroxyl radicals. In addition, GSLS can alleviate chlorpyrifos-induced ROS accumulation in L02 cells, alleviating cytokinetic potential reduction. In summary, by fighting oxidative stress, GSLS can alleviate liver damage caused by CPF.

中文翻译：

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Ros介导的线粒体氧化应激参与人参皂苷GSLS对毒死蜱诱导的小鼠肝毒性的改善作用

毒死蜱 (CPF) 作为一种广泛使用的有机磷农药，经常残留在食物表面或污染水源。CPF会对人类的生产和生活造成诸多毒害。人参茎叶总皂苷(GSLS)作为人参非药用部位的附加产品，其药理活性近年来得到验证和应用。本研究旨在评估 GSLS 对 CPF 诱导的小鼠肝损伤的保护作用。体内实验结果证明 GSLS 可以通过减轻 CPF 诱导的小鼠肝功能指标和增强小鼠抗氧化酶 SOD 和 CAT 活性来减少氧化产物 MDA 的积累。随着肝组织中BAX、NF-KB、TIMP mRNA表达的降低，Nrf-2、HO-1、XIAP的mRNA表达升高。GSLS可通过抗炎、抗氧化、抗炎作用减轻CPF引起的肝毒性。体外实验证明GSLS能显示出清除DPPH自由基和羟基自由基的能力。此外，GSLS 可以减轻毒死蜱诱导的 L02 细胞 ROS 积累，减轻细胞因子潜能降低。总之，通过对抗氧化应激，GSLS 可以减轻 CPF 引起的肝损伤。