This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers. A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each. Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides. The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG. Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers. Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways. Moreover, there was a close correlation between serum metabolites, cecal microbiota and phenotypic indicators. Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.

中文翻译：

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基于血清代谢组学和盲肠微生物群的黄芪甘草复合多糖对肉鸡生长性能、肉质及抗氧化功能的影响

本研究旨在评估日粮添加黄芪和甘草复合多糖对肉鸡生长性能、肉质、抗氧化功能、盲肠微生物群和血清代谢组学的影响。总共 480 只 1 日龄雄性 Arbor Acres (AA) 肉鸡被随机分成 4 个处理，每个处理 6 个重复，每组 20 只肉鸡。治疗：CON组为基础饮食；ANT组补充土霉素钙；LAG组补充黄芪多糖150mg/kg、甘草多糖75mg/kg ；HAG组补充黄芪多糖300 mg/kg和甘草150 mg/kg多糖。结果表明，与CON组和ANT组相比，LAG和HAG的补充提高了生长性能、抗氧化功能和肉质，尤其是LAG处理的效果优于HAG。对盲肠微生物群的分析表明，添加 LAG 和 HAG 改变了肉鸡的盲肠微生物多样性和组成。血清代谢组学分析显示，CON组和LAG组共鉴定出193种差异代谢物，主要富集亚油酸代谢和谷胱甘肽代谢途径。此外，血清代谢物、盲肠微生物群和表型指标之间存在密切相关性。结论：日粮添加150 mg/kg黄芪多糖和75 mg/kg甘草多糖可提高生长性能，