Perchlorate is a known endocrine-disrupting micropollutant. The efficiency of a low-cost in-situ bio-remediation process for perchlorate-contaminated aqueous phase was evaluated in a bench-scale unit in this study. The two-stage process unit comprises an anaerobic leach bed unit (5.3 L) for generating leachate and an anaerobic filter bed unit (10 L) inoculated with an isolated perchlorate reducing Serratia marcescens (GenBank Accession No. JQ807993). Organic leachate produced from anaerobic digestion of vegetable waste served as a sole substrate for the perchlorate reduction, and needle-felt natural fibre was used as a filter bed medium. The filter bed unit removed 98.5% of perchlorate at 10 mg/L initial concentration (volumetric loading, 39 mg/L/day) at an optimal soluble COD concentration of 40 mg/L in the leachate and a hydraulic retention time of 6.15 h. Controlled leachate delivery results in an effluent COD < 20 mg/L, reducing the risk of residual organic contamination in the treated water. Considering the many advantages, this approach would be more feasible for treating perchlorate-contaminated aquifers, streams, and surface canals.

高氯酸盐是一种已知的内分泌干扰微污染物。在本研究中，对高氯酸盐污染水相的低成本原位生物修复工艺的效率进行了评估。两阶段工艺单元包括用于产生渗滤液的厌氧浸出床单元（5.3 L）和接种了分离的高氯酸盐还原粘质沙雷氏菌的厌氧过滤床单元（10 L）(GenBank 登录号 JQ807993)。植物废物厌氧消化产生的有机渗滤液作为高氯酸盐还原的唯一基质，针毡天然纤维用作滤床介质。滤床单元去除了 98.5% 的高氯酸盐，初始浓度为 10 mg/L（容积负荷，39 mg/L/天），渗滤液中的最佳可溶性 COD 浓度为 40 mg/L，水力停留时间为 6.15 h。受控的渗滤液输送导致出水 COD < 20 mg/L，从而降低了处理水中残留有机污染物的风险。考虑到许多优点，这种方法对于处理被高氯酸盐污染的含水层、溪流和地表运河更可行。