As an ecological niche close to the polymer, microorganisms in the plastisphere possess the advantage of degrading plastics. This study aims to investigate the bacterial community succession and obtain degrading bacteria in the plastisphere, as well as identify the most efficient degradation combination by co-culture of multiple strains. The findings demonstrate the alpha-diversity indices of the plastisphere bacterial community are significantly lower, and the community structure is regularly and significantly altered. With the time of culture, the plastisphere community composition alters regularly, and the hydrocarbon-degrading genera become the core members. Functional prediction of community reveals the potential for Xenobiotics Biodegradation and Metabolism of plastisphere, and the apparent variations detections of polyethylene mulching film (PMF) indicating the PMF degrading ability of plastisphere. Besides, three PMF-degrading bacterial strains, Rhodopseudomonas sp. P1 (P), Rhodanobacter sp. Rs (R) and Microbacterium sp. M1 (M), are screened for co-culture with PMF degrading strain Bacillus aryabhattai 5–3 (B). By considering bacterial growth, biofilm adhesion, and apparent degradation of different samples, RB (R. sp. Rs + B. aryabhattai 5–3) is ultimately selected as the best PMF degradation combination. This study provides a new possibility for plastisphere-related research from the perspective of mitigating plastic pollution on agricultural land.

作为接近聚合物的生态位，塑料球中的微生物具有降解塑料的优势。本研究旨在调查细菌群落演替并获得塑料球中的降解细菌，并通过多种菌株的共培养确定最有效的降解组合。研究结果表明，塑料球细菌群落的α多样性指数显着降低，群落结构经常发生显着变化。随着文化的发展，塑性圈群落组成有规律地变化，烃类降解属成为核心成员。群落的功能预测揭示了塑料球外来生物降解和代谢的潜力，以及聚乙烯地膜（PMF）的表观变化检测表明塑料球对PMF的降解能力。此外，三种 PMF 降解菌株，红假单胞菌属 P1（P），Rhodanobacter sp。Rs (R) 和Microbacterium sp。筛选 M1 (M) 与 PMF 降解菌株Bacillus aryabhattai 5-3 (B) 共培养。通过考虑不同样品的细菌生长、生物膜粘附和表观降解，最终选择 RB ( R. sp. Rs + B. aryabhattai 5-3) 作为最佳 PMF 降解组合。本研究从减轻农业用地塑料污染的角度为塑料圈相关研究提供了新的可能。