Peptide mimotopes to emulate carbohydrates,Chemical Society Reviews

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Peptide mimotopes to emulate carbohydrates
Chemical Society Reviews ( IF 40.4 ) Pub Date : 2022-09-21 , DOI: 10.1039/d2cs00470d
Teruhiko Matsubara ₁

Affiliation

Glycoconjugates on animal cell surfaces are involved in numerous biological functions and diseases, especially the adhesion/metastasis of cancer cells, infection, and the onset of glycan-related diseases. In addition to glycoantigen detection, the regulation of glycan (carbohydrate)–protein interactions is needed to develop therapeutic strategies for glycan-related diseases. Preparation of a diverse range of glycan derivatives requires a massive effort, but the preparation and identification of alternative glycan-mimetic peptide mimotopes may provide a solution to this issue. Peptide mimotopes are recognized by glycan-binding proteins, such as lectins, enzymes, and antibodies, alternative to glycan ligands. Phage-display technology is the first choice in the selection of “glycan (carbohydrate)-mimetic peptide mimotopes” from a large repertoire of library sequences. This tutorial review describes the advantages of peptide mimotopes in comparison to glycan ligands, as well as their structural and functional mimicry. The detailed library design is followed by a description of the strategy used to improve affinity, and finally, an outline of the vaccine application of glycan-mimetic peptides is provided.

中文翻译：

模拟碳水化合物的肽模拟表位

动物细胞表面的糖缀合物与许多生物学功能和疾病有关，尤其是癌细胞的粘附/转移、感染和聚糖相关疾病的发作。除了糖抗原检测之外，还需要调节聚糖（碳水化合物）-蛋白质相互作用来制定聚糖相关疾病的治疗策略。制备多种聚糖衍生物需要付出巨大的努力，但替代聚糖模拟肽模拟表位的制备和鉴定可能会为这个问题提供解决方案。肽模拟表位被聚糖结合蛋白识别，例如凝集素、酶和抗体，可替代聚糖配体。噬菌体展示技术是从大量文库序列中选择“聚糖（碳水化合物）-模拟肽模拟表位”的首选技术。本教程回顾描述了肽模拟表位与聚糖配体相比的优势，以及它们的结构和功能模拟。在详细的文库设计之后，描述了用于提高亲和力的策略，最后，提供了聚糖模拟肽疫苗应用的概述。

更新日期：2022-09-21

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