The aim of this study was to monitor the effect of Bifidobacterium bifidum (BB) and the combination of Lactobacillus sporogenes, Enterococcus faecium, and Bifidobacterium bifidum (LEB) on the health status and weight gain of calves, and the utilisation of nitrogenous substances. The experiment was performed in the period from April 2020 to September 2020. A total of 90 Holstein heifers, which were one to 56 days old, were used as experimental animals. Differences in live weight gain were significant if we compared the LEB vs BB group and the LEB vs C, the control group (86.23 ± 5.49 kg vs. 84.72 ± 6.22 kg, p <0.05; 86.23 ± 5.49kg vs. 82.86 ± 5.35 kg, p < 0.01). Considering the live weight gain, group BB was heavier than group C only (84.72 ± 6.22 kg vs. 82.86 ± 5.35 kg, p <0.05). An effect on reducing the incidence and duration of diarrheal diseases was not demonstrated in this study (p = 0.1957). The administration of feed additives had no statistically significant effect on the amount of N excreted in the feces. The values of hematological and biochemical parameters were unaffected except for the first sampling of urea. Other blood parameters were not affected by the addition of probiotic feed additives. The bacterial populations in the feces 5 days and 56 days after birth were not affected by the inclusion of feed additives.

中文翻译：

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益生菌菌株双歧杆菌、乳杆菌和肠球菌对犊牛健康状况和体重增加的影响，以及含氮化合物的利用

本研究的目的是监测双歧双歧杆菌(BB) 和产孢乳杆菌、粪肠球菌和双歧双歧杆菌(LEB)的组合对犊牛健康状况和增重以及含氮物质利用的影响。实验时间为2020年4月至2020年9月，共90头1至56日龄的荷斯坦小母牛作为实验动物。如果我们比较 LEB 与 BB 组和 LEB 与 C，对照组（86.23 ± 5.49 kg 与 84.72 ± 6.22 kg，p <0.05；86.23 ± 5.49 kg 与 82.86 ± 5.35 kg） ,磷< 0.01)。考虑到活重增加，BB 组仅比 C 组重（84.72 ± 6.22 公斤对 82.86 ± 5.35 公斤，p < 0.05）。本研究未证明对减少腹泻病的发病率和持续时间有影响 ( p = 0.1957)。饲料添加剂的施用对粪便中排泄的 N 量没有统计学显着影响。除了尿素的第一次采样外，血液学和生化参数的值未受影响。其他血液参数不受添加益生菌饲料添加剂的影响。出生后 5 天和 56 天粪便中的细菌数量不受添加饲料添加剂的影响。