Transmission of pathogenic microorganisms in the last decades has been considered a significant health hazard and pathogenic E. coli, particularly antibiotic-resistant strains, have long been identified as a zoonotic problem. This study aimed to investigate multidrug resistant pathogenic E. coli isolates from wild birds, chickens, and environment in selected Orang Asli and Malay villages in Peninsular Malaysia. The bacteriological culture-based technique, disc diffusion method, and multiplex Polymerase Chain Reaction (mPCR) assay was used to determine the occurrence of pathogenic E. coli strains in the several samples in the study. E. coli isolates showed a variety of multi-drug resistant (MDR) antibiotypes and Enteropathogenic E. coli (EPEC) and Enteroinvasive E. coli (EIEC) were the most predominantly identified pathogenic E. coli strains. The findings of this study demonstrated the significance of animal reservoirs and the environment as sources of pathogenic E. coli, resistant bacteria, and resistance genes. Hence, there is a need for adoption of a practical surveillance approach on MDR pathogens to control foodborne contamination.

中文翻译：

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从马来西亚野生鸟类、鸡和环境中分离出的多重耐药致病性大肠杆菌


过去几十年来，病原微生物的传播被认为是一种重大的健康危害，而病原性大肠杆菌，特别是耐抗生素菌株，长期以来一直被认为是人畜共患问题。本研究旨在调查马来西亚半岛选定的原住民和马来村庄的野鸟、鸡和环境中分离出的多重耐药致病性大肠杆菌。基于细菌学培养的技术、纸片扩散法和多重聚合酶链反应 (mPCR) 测定用于确定研究中多个样品中致病性大肠杆菌菌株的出现。大肠杆菌分离株显示出多种多重耐药 (MDR) 抗体型，肠致病性大肠杆菌(EPEC) 和肠侵袭性大肠杆菌(EIEC) 是最主要的致病性大肠杆菌菌株。这项研究的结果证明了动物宿主和环境作为致病性大肠杆菌、耐药细菌和耐药基因来源的重要性。因此，有必要对耐多药病原体采取实用的监测方法来控制食源性污染。