Objectives: The inherent toxicities of the drugs urge the search for alternative drugs that are safe and effective. Therefore, the objective of the study is to evaluate the in-vivo anti-diarrheal activity of crude extract and solvent fractions of Rhamnus prinoides leaves. Methods: The Leaves of Rhamnus prinoides were macerated using absolute methanol and then fractionated. For in-vivo antidiarrheal activity evaluation of the crude extract and solvent fraction, castor oil-induced diarrhea, castor oil-induced anti-enteropolling, and intestinal transit models were used. One-way analysis of variance was used to analyze the data, followed by a Tukey post-test. The standard and negative control groups were treated with loperamide and 2% tween 80 respectively. Results: A significant reduction in the frequency of wet stools and watery content of diarrhea, intestinal motility, intestinal fluid accumulation, and delaying the onset of diarrhea as compared with controls were observed in mice treated with 200 mg/kg and 400 mg/kg ME. However the effect increased dose-dependently, and the 400 mg/kg ME produced a comparable effect with the standard drug in all models. Amongst the solvent fractions, n-BF significantly delayed the time of diarrheal onset and reduced the frequency of defecation, and intestinal motility at doses of 200mg/kg and 400mg/kg. Furthermore, the maximum percentage inhibition of intestinal fluid accumulation was observed in mice treated with 400 mg/kg n-BF (p<0.01; 61.05%). Conclusion: The results of this study showed that crude leaves extract and solvent fractions of Rhamnus prinoides had significant anti-diarrheal activity, providing scientific support for its traditional use as a diarrhea treatment.

中文翻译：

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体内止泻活性：来自 Rhamnus prinoides (Rhamnaceae) 叶子的粗提物和溶剂部分

目的：药物的固有毒性促使人们寻找安全有效的替代药物。因此，本研究的目的是评估鼠李叶粗提物和溶剂部分的体内抗腹泻活性。方法：用无水甲醇浸渍鼠李叶，然后分级分离。对于粗提物和溶剂部分的体内抗腹泻活性评估，使用蓖麻油诱导的腹泻、蓖麻油诱导的抗肠花粉和肠道转运模型。使用单向方差分析来分析数据，然后进行 Tukey 后测。标准组和阴性对照组分别用洛哌丁胺和2%吐温80处理。结果：湿便频率和腹泻水样含量显着减少，与对照组相比，在用 200 mg/kg 和 400 mg/kg ME 治疗的小鼠中观察到肠蠕动、肠液积聚和延迟腹泻发作。然而，效果呈剂量依赖性增加，并且 400 mg/kg ME 在所有模型中产生与标准药物相当的效果。在溶剂组分中，n-BF 在 200mg/kg 和 400mg/kg 的剂量下显着延迟了腹泻发作时间并降低了排便频率和肠蠕动。此外，在用 400 mg/kg n-BF 处理的小鼠中观察到肠液积聚的最大百分比抑制 (p<0.01; 61.05%)。结论：本研究结果表明，鼠李的粗叶提取物和溶剂组分具有显着的抗腹泻活性，