UV–Visible spectrophotometers are widely used in academia, research and industry for the product verification and quality assurance. The regular performance check of spectrophotometer is required for quality assurance and can be done by performing the calibration periodically using specific methods or reference materials. We have prepared 4% Ho₂O₃ solution in perchlorate acid as an indigenous reference material for wavelength calibration of spectrophotometer. The transmission spectra for Ho₂O₃ solution have been collected in the wavelength range of 200–700 nm. Role of selected parameters such as solution concentration and spectral band width on band position has been investigated. A total of 19 transmittance bands were observed, and out of them, 11 bands are considered for wavelength calibration. The associated uncertainty has been evaluated for all 11 transmission bands. The associated uncertainty is found to be within ± 1.5 nm with coverage factor 2 at 95% confidence level.

紫外-可见分光光度计广泛用于学术界、研究和工业界，用于产品验证和质量保证。分光光度计的定期性能检查是质量保证所必需的，可以通过使用特定方法或参考材料定期进行校准来完成。我们制备了 4% Ho ₂ O ₃的高氯酸溶液作为分光光度计波长校准的本土参考材料。Ho ₂ O _{3的透射光谱}溶液已在 200-700 nm 的波长范围内收集。研究了所选参数（例如溶液浓度和光谱带宽）对波段位置的作用。共观察到 19 个透射波段，其中 11 个波段被考虑用于波长校准。已对所有 11 个传输频段的相关不确定性进行了评估。发现相关的不确定性在 ± 1.5 nm 范围内，覆盖因子为 2，置信度为 95%。