Activity, selection response and molecular mode of action of the isoxazoline afoxolaner in Tetranychus urticae,Pest Management Science

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Activity, selection response and molecular mode of action of the isoxazoline afoxolaner in Tetranychus urticae
Pest Management Science ( IF 3.8 ) Pub Date : 2022-09-18 , DOI: 10.1002/ps.7187
Catherine Mermans ₁ , Wannes Dermauw _{1,

2} , Sven Geibel ₃ , Thomas Van Leeuwen ₁

Affiliation

Afoxolaner is a novel representative of the isoxazolines, a class of ectoparasiticides which has been commercialized for the control of tick and flea infestations in dogs. In this study, the biological efficacy of afoxolaner against the two-spotted spider mite Tetranychus urticae was evaluated. Furthermore, as isoxazolines are known inhibitors of γ-aminobutyric acid-gated chloride channels (GABACls), the molecular mode of action of afoxolaner on T. urticae GABACls (TuRdls) was studied using functional expression in Xenopus oocytes followed by two-electrode voltage-clamp (TEVC) electrophysiology, and results were compared with inhibition by fluralaner, fipronil and endosulfan. To examine the influence of known GABACl resistance mutations, H301A, I305T and A350T substitutions in TuRdl1 and a S301A substitution in TuRdl2 were introduced.

中文翻译：

异恶唑啉 afoxolaner 在 Tetranychus urticae 中的活性、选择反应和分子作用模式

Afoxolaner 是异恶唑啉类的新型代表，异恶唑啉类是一类外寄生虫剂，已商业化用于控制狗的蜱虫和跳蚤感染。在这项研究中，评估了 afoxolaner 对二斑叶螨Tetranychus urticae的生物学功效。此外，由于异恶唑啉是已知的 γ-氨基丁酸门控氯离子通道 (GABACls) 抑制剂，因此使用非洲爪蟾中的功能表达研究了 afoxolaner 对T. urticae GABACls ( Tu Rdls) 的分子作用模式卵母细胞随后进行双电极电压钳 (TEVC) 电生理学，并将结果与 fluralaner、氟虫腈和硫丹的抑制作用进行比较。为了检查已知的 GABACl 抗性突变的影响，引入了 Tu Rdl1 中的 H301A、I305T 和 A350T 取代以及Tu Rdl2中的 S301A 取代。

更新日期：2022-09-18

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