Microwave-assisted dispersive liquid–liquid microextraction (MADLLME) coupled with high-performance liquid chromatography (HPLC) with diode array detector was used for the extraction and determination of three biogenic amines (BAs), including tryptamine, histamine and phenylethylamine in beverages (beer, cherry juice and white spirit). Compared with solid-phase extraction, solid-phase microextraction and liquid-phase microextraction, which is more solvent use with lower extraction efficiency, this MADLLME method obviously shortened analytical time, the rapid heating of aqueous samples with non-ionizing electromagnetic radiation, a lower solvent use and enhanced extraction efficiency. Because of good extraction for three BAs, [3C6PC14][FeCl4] was used as an extraction solvent. We showed a tunable selectivity of magnetic ionic liquids (MILs) toward extracting BAs by changing anion or cation due to the modification of the interaction between the MIL and the BAs. Extraction conditions including the type and volume of extraction solvent, microwave power, microwave-assisted extraction time, sample pH, disperser and interference experiment were investigated. Under the optimal conditions, a good linear relationship was found in the concentration range of 100–2,000 ng mL−1 for three BAs with correlation coefficient (R2) of 0.995–0.999. The limit of detections (S/N = 3) and limit of quantitations (S/N = 10) were in the range of 3.46–4.96 ng mL−1 and 10.44–14.88 ng mL−1, respectively. The recoveries of three targets were in the range of 84.3–108.5%, and the relative standard deviations based on the peak areas for six replicate analyses of beverages spiked with 10, 50 and 100 ng mL−1 of each biogenic amine were lower than 7.9%. This method has also been successfully applied to analyze the real samples at three different spiked concentrations, and excellent results have been obtained.

中文翻译：

---

微波辅助分散液-液微萃取结合高效液相色谱法测定饮料中的三种生物胺

采用微波辅助分散液液微萃取（MADLLME）与带二极管阵列检测器的高效液相色谱（HPLC）联用，对饮料（啤酒）中的色胺、组胺和苯乙胺等三种生物胺（BA）进行提取和测定。 、樱桃汁和白酒）。与固相萃取、固相微萃取和液相微萃取等溶剂用量较多、萃取效率较低的方法相比，MADLLME方法明显缩短了分析时间，利用非电离电磁辐射对水样进行快速加热，降低了萃取效率。溶剂的使用和提高萃取效率。由于对三种 BA 的萃取效果良好，因此使用 [3C6PC14][FeCl4] 作为萃取溶剂。由于 MIL 和 BA 之间相互作用的改变，我们通过改变阴离子或阳离子，展示了磁性离子液体 (MIL) 对提取 BA 的可调选择性。考察了提取溶剂的种类和体积、微波功率、微波辅助提取时间、样品pH、分散器和干扰实验等提取条件。在最佳条件下，三种BA在100-2,000 ng mL−1的浓度范围内呈良好的线性关系，相关系数（R2）为0.995-0.999。检测限 (S/N = 3) 和定量限 (S/N = 10) 分别在 3.46–4.96 ng mL−1 和 10.44–14.88 ng mL−1 范围内。三个目标的回收率在 84.3–108.5% 范围内，对添加了 10、50 和 100 ng mL−1 每种生物胺的饮料进行六次重复分析，基于峰面积的相对标准偏差低于 7.9 %。该方法还成功应用于分析三种不同加标浓度的实际样品，并获得了良好的结果。