Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases.

淀粉样斑块或原纤维是异常蛋白质的沉积物，主要含有β-折叠，它们在不同的器官和组织中积聚，导致组织功能受损并导致致命和衰弱的疾病。在这项研究中，鼠尾草的作用研究了从其花制备的提取物（SOE）对预防淀粉样蛋白斑形成和去除积聚原纤维的作用。因此，在不存在和存在 7、14 和 28 mg/ml SOE 的情况下，在高温和酸性 pH 条件下孵育 14 mg/ml 的鸡蛋清溶菌酶 (HEWL)，以形成淀粉样蛋白原纤维。在该系列中，采用硫代黄素 T (THT)、1-苯胺萘-8-磺酸 (ANS) 荧光试验、刚果红吸光度试验、圆二色性和场发射扫描电子显微镜研究了提取物对这些物质的影响。过程。结果表明，SOE 具有很强的抗淀粉样蛋白生成活性。0.5:1 (7:14 mg/ml) 的提取物与蛋白质的比例不会导致在 HEWL 溶液中形成淀粉样蛋白原纤维。然而，它以剂量依赖的方式导致 HEWL 的除颤。根据我们的结果，提取物与蛋白质的比例为 1:1 (14:14 mg/ml) 是将长而大的 HEWL 原纤维结构改变为细而小的原纤维的最佳比例。一般而言，可以得出结论，SOE 含有可用于治疗淀粉样蛋白依赖性疾病的化合物。