The application of surface-enhanced Raman scattering (SERS) spectroscopy to ultrasensitively and quantitatively analyze small molecules of interest in biological samples has been a challenging endeavor, on account of the signal contamination caused by a variety of macromolecules including lipids, proteins and nucleic acids. Herein, we designed a “three-in-one” multifunctional substrate (Au ETHH@ZIF-8) composed of Au elongated tetrahexahedron (Au ETHH) and zeolitic imidazolate framework-8 (ZIF-8) with analyte enrichment, filtration and enhanced SERS performance for the detection of 4-nitrobenzenethiol (4-NBT). Au ETHH nanocrystals with a high density of sharp edges and tips enhance SERS performance. The ZIF-8 shell with the porous structure surrounding the Au ETHH, allows those analytes of comparable or smaller size to the pore size to reach the surface of the Au ETHH and possesses moderate analyte enrichment property. In addition, the prepared Au ETHH@ZIF-8-based SERS sensor realized the ultrasensitive and quantitative detection of 4-NBT in whole blood.

由于包括脂质、蛋白质和核酸在内的多种大分子引起的信号污染，应用表面增强拉曼散射 (SERS) 光谱对生物样品中感兴趣的小分子进行超灵敏和定量分析一直是一项具有挑战性的工作。在此，我们设计了一种“三合一”多功能基底（Au ETHH@ZIF-8），由金细长四面体（Au ETHH）和沸石咪唑骨架 8（ZIF-8）组成，具有分析物富集、过滤和增强的 SERS用于检测 4-硝基苯硫醇 (4-NBT) 的性能。具有高密度锋利边缘和尖端的 Au ETHH 纳米晶体增强了 SERS 性能。具有围绕 Au ETHH 的多孔结构的 ZIF-8 壳，允许那些尺寸与孔径相当或更小的分析物到达Au ETHH的表面并具有中等的分析物富集特性。此外，所制备的基于Au ETHH@ZIF-8的SERS传感器实现了全血中4-NBT的超灵敏定量检测。