Background

Gestational diabetes mellitus (GDM) is characterized by insulin resistance during pregnancy, and it is always combined with serious complications. Dendrobium mixture (DMix) is a kind of traditional Chinese medicine, and it has been proved to be an effective treatment for diabetes. However, the regulatory role of DMix in GDM remains elusive.

Methods

High fat feed combined with streptozotocin injection and high glucose medium were used to establish GDM animal and cell models, respectively. The levels of blood glucose, blood lipid, and insulin were measured with commercial kits. Western blotting was used to detect protein expression.

Results

DMix improved pancreas and placenta injury in GDM rats. DMix reversed the influence of GDM on the levels of SOD, MDA, and glutathione in the serum. Hyperglycemia and hyperlipidemia in GDM rats were suppressed by DMix. The activation of MAPK and inhibition of Nrf2/HO1 in GDM animal and cell models were reversed by DMix. The increase of ROS intensity, apoptosis, and inflammation factors in HG treated cells were reversed by DMix.

Conclusion

This research proved that DMix improved GDM through inhibiting oxidative condition, inflammation factors, hyperglycemia and hyperlipidemia. This study might provide a novel thought for the prevention and treatment of GDM.

中文翻译：

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石斛合剂调控Nrf2/HO1信号通路改善妊娠糖尿病

背景

妊娠期糖尿病（GDM）以孕期胰岛素抵抗为特征，常伴有严重并发症。石斛合剂（DMix）是一种中药，已被证明是治疗糖尿病的有效药物。然而，DMix 在 GDM 中的调节作用仍然难以捉摸。

方法

采用高脂饲料联合链脲佐菌素注射液和高糖培养基分别建立GDM动物和细胞模型。用商业试剂盒测量血糖、血脂和胰岛素水平。蛋白质印迹用于检测蛋白质表达。

结果

DMix 改善了 GDM 大鼠的胰腺和胎盘损伤。DMix 逆转了 GDM 对血清中 SOD、MDA 和谷胱甘肽水平的影响。DMix 抑制了 GDM 大鼠的高血糖和高脂血症。DMix 逆转了 GDM 动物和细胞模型中 MAPK 的激活和 Nrf2/HO1 的抑制。DMix 逆转了 HG 处理细胞中 ROS 强度、细胞凋亡和炎症因子的增加。

结论

该研究证明，DMix通过抑制氧化状态、炎症因子、高血糖和高脂血症来改善GDM。本研究可能为GDM的防治提供一种新思路。