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Isolation, chemical characterization and in vitro bioactive potential of polysaccharides from seaweed Portieria hornemannii
Biomass Conversion and Biorefinery ( IF 3.5 ) Pub Date : 2022-09-16 , DOI: 10.1007/s13399-022-03276-5
Manickam Elangovan , Perumal Anantharaman , M. Kavisri , Meivelu Moovendhan

The goal of this work is to isolate biopolymers from P. hornemannii and assess their chemical composition, structural characteristics and possible antibacterial, antioxidant and anti-diabetic activities. Seaweed P. hornemannii was used to extract the polysaccharides, which were determined to be 34% and contained 1.50% nitrogen, 20.55% carbon and 3.23% of hydrogen. The chemical components such as total carbohydrate, moisture and ash were estimated in polysaccharide from seaweed P. hornemannii and the results were found to be 71, 8 and 3%, respectively. The active functional groups alkyl halide (C–Br), alkyl halide (C-F), alkane (-C-H), alkene (= C-H) and alcohol (O–H) were recorded at the peak between 455.20 and 3429.43 cm−1 by IR spectra and –OH alcohol monomeric, alkyne methine and acetal protons were observed in proto NMR, respectively. In SEM analysis cross linkage, rough surface with porosity and voids surface morphology has been noted. The antibiotic potential of polysaccharides was studied against clinical pathogens and showed maximum activity of 21 and 18 mm zone of inhibition against Vibrio cholerae and Staphylococcus aureus strains at 600 µg/ml concentrations. In antioxidant scavenging screening, the polysaccharide has shown a promising scavenging effect against total antioxidant, DPPH, ferrous chelating and hydroxyl radicals in vitro assays in higher concentrations. The potential antidiabetic effects of polysaccharides were revealed in 79% and 61% α-amylase and β-glucosidase enzymes inhibition at 1000 µg/ml. Seaweed polysaccharide can be used in pharmaceutical industry also as novel multifunctional drug.



中文翻译:

海藻 Portieria hornemannii 多糖的分离、化学表征和体外生物活性潜力

这项工作的目标是从P. hornemannii中分离出生物聚合物,并评估它们的化学成分、结构特征和可能的抗菌、抗氧化和抗糖尿病活性。海藻P. hornemannii用于提取多糖,经测定其含量为 34%,含有 1.50% 的氮、20.55% 的碳和 3.23% 的氢。对海藻P. hornemannii多糖中的总碳水化合物、水分和灰分等化学成分进行了估算,结果分别为 71%、8% 和 3%。活性官能团卤代烷 (C-Br)、卤代烷 (CF)、烷烃 (-CH)、烯烃 (= CH) 和醇 (O-H) 记录在 455.20 和 3429.43 cm -1之间的峰值处通过红外光谱和-OH 醇单体,在原核磁共振中分别观察到炔烃和乙缩醛质子。在 SEM 分析交联时,已经注意到具有孔隙率和空隙表面形态的粗糙表面。研究了多糖对临床病原体的抗生素潜力,并显示出对霍乱弧菌金黄色葡萄球菌的 21 和 18 毫米抑制区的最大活性浓度为 600 µg/ml 的菌株。在抗氧化剂清除筛选中,多糖在较高浓度的体外测定中显示出对总抗氧化剂、DPPH、亚铁螯合和羟基自由基的有希望的清除作用。多糖的潜在抗糖尿病作用在 1000 µg/ml 时可抑制 79% 和 61% 的 α-淀粉酶和 β-葡萄糖苷酶。海藻多糖可用于医药工业,也可作为新型多功能药物。

更新日期:2022-09-17
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