Abstract

With the introduction of the Sysmex XN-10, new platelet indices reflecting platelet maturity can be obtained alongside with a full blood count. Therefore, the need for verified reference intervals is present. The purpose of this study was to establish reference intervals for the platelet indices: platelet large cell ratio (P-LCR), platelet distribution width (PDW) and plateletcrit (PCT) in a large Scandinavian cohort. Furthermore, we aimed to verify previously established reference intervals of haematological parameters included in a full blood count. Blood samples were obtained from healthy Danish blood donors and analysed by use of the Sysmex XN-10 analyser (Sysmex, Kobe, Japan). Non-parametric 95% reference intervals were determined as the 2.5 and 97.5 percentile and presented with 90% confidence intervals (CI). In total, 30,917 blood donors were included and the following reference intervals were established: P-LCR, ratio: 17.3 (90% CI: 17.2 – 17.5) – 46.2 (90% CI:45.9 – 46.4); P DW, fL:9.5 (90% CI: 9.5 – 9.5) – 17.2 (90% CI: 17.2 – 17.3) and PCT, fraction: 0.18 (0.18 – 0.18) – 0.38 (0.38 – 0.39). The reference intervals were stable across age and sex. Furthermore, reference intervals for the remaining haematological parameters included in a full blood count were verified and found in line with the previously established reference intervals.

摘要

随着 Sysmex XN-10 的推出，可以在全血细胞计数的同时获得反映血小板成熟度的新血小板指数。因此，需要验证参考区间。本研究的目的是在斯堪的纳维亚大型队列中建立血小板指数的参考区间：血小板大细胞比率 (P-LCR)、血小板分布宽度 (PDW) 和血小板比容 (PCT)。此外，我们旨在验证先前建立的全血细胞计数中血液学参数的参考区间。血液样本取自健康的丹麦献血者，并使用 Sysmex XN-10 分析仪（Sysmex，Kobe，Japan）进行分析。非参数 95% 参考区间被确定为 2.5 和 97.5 百分位数，并以 90% 置信区间 (CI) 表示。总共30个，纳入了 917 名献血者，并建立了以下参考区间：P-LCR，比率：17.3（90% CI：17.2 - 17.5） - 46.2（90% CI：45.9 - 46.4）；P DW, fL:9.5 (90% CI: 9.5 – 9.5) – 17.2 (90% CI: 17.2 – 17.3) 和 PCT，分数：0.18 (0.18 – 0.18) – 0.38 (0.38 – 0.39)。参考区间在年龄和性别上是稳定的。此外，对全血细胞计数中包含的其余血液学参数的参考区间进​​行了验证，并发现与先前建立的参考区间一致。