Pomegranate peel extract (PPE) has been widely identified as a rich source of therapeutically active phyto-compounds, known for their beneficial effects in aquaculturable species. In this context, a 60-day feeding trial was carried out to evaluate the effect of supplementation of pomegranate peel extract (PPE) on growth performance, haemato-immunological responses and cytokine gene expression in Labeo rohita (rohu) fingerlings challenged with Aeromonas hydrophila. A total of 240 rohu fingerlings (3.25 ± 0.52 g) were randomly distributed into four treatment groups in triplicates with 15 fish per tank. The groups were fed with four different diets containing graded levels of PPE viz., 0 (control), 0.5, 1.0 and 1.5%. The results demonstrated significantly enhanced weight gain% and specific growth rate (SGR) in the group fed with 0.5% of dietary PPE. The cumulative survival post challenge to A. hydrophila was enhanced to 72.90% in 0.5% PPE fed group as compared to 20.01% in the control group. Haemato-immunological parameters in terms of respiratory burst activity (NBT), serum total protein, globulin and lysozyme activity displayed highest levels in L. rohita fed with the 0.5% PPE diet. Further, the dietary group supplemented with PPE at 0.5% level exhibited upregulated mRNA transcript levels of pro-inflammatory cytokines viz. IL-1β, TNF-α in different lymphoid organs such as gut, kidney and liver of L. rohita fingerlings post-challenge. In contrast, anti-inflammatory cytokine IL-10, was significantly down-regulated in infected rohu fed with 0.5% PPE. Taken together, the best optimum dosage or inclusion level at 0.5% PPE, obtained through the study, represented remarkable favourable variations in growth, haemato-immunological parameters and cytokine gene expression of L. rohita fingerlings. Thus, the dietary supplementation of PPE at 0.5% could effectively enhance different aspects of innate immunity alongwith disease resistance to A. hydrophila infection in L. rohita fingerlings.

石榴皮提取物 (PPE) 已被广泛认为是具有治疗活性的植物化合物的丰富来源，因其对水产养殖物种的有益作用而闻名。在此背景下，进行了为期 60 天的饲养试验，以评估补充石榴皮提取物 (PPE) 对嗜水气单胞菌攻击的Labeo rohita (rohu) 鱼种生长性能、血液免疫反应和细胞因子基因表达的影响. 将总共​​ 240 条罗胡鱼种 (3.25 ± 0.52 g) 随机分配到四个处理组，一式三份，每缸 15 条鱼。给这些组喂食四种不同的饮食，这些饮食含有分级水平的 PPE，即 0（对照）、0.5、1.0 和 1.5%。结果表明，饲喂 0.5% 膳食 PPE 的组显着提高了体重增加百分比和比生长率 (SGR)。与对照组中的 20.01% 相比，在 0.5% PPE 喂养组中对 A.hydrophila 攻击后的累积存活率提高到 72.90 %。呼吸爆发活性 (NBT)、血清总蛋白、球蛋白和溶菌酶活性方面的血液免疫学参数在L. rohita中表现出最高水平喂食 0.5% PPE 饮食。此外，补充有 0.5% 水平的 PPE 的饮食组表现出上调的促炎细胞因子的 mRNA 转录水平，即。L. rohita鱼种攻击后不同淋巴器官如肠道、肾脏和肝脏中的 IL-1β、TNF-α 。相比之下，抗炎细胞因子 IL-10 在喂食 0.5% PPE 的感染罗胡中显着下调。综上所述，通过该研究获得的 0.5% PPE 的最佳最佳剂量或包含水平代表了L. rohita鱼种的生长、血液免疫学参数和细胞因子基因表达的显着有利变化。因此，膳食补充 0.5% 的 PPE 可以有效增强先天免疫的不同方面以及抗病能力。L. rohita鱼种中的A.hydrophila感染。