Sulfidic mine waste usually contains elevated amounts of valuable and hazardous metal(loid)s, which may pose environmental risks but can also provide opportunities for resource recovery. Reprocessing of mine waste can offer both economic and environmental benefits by supplying some of the ever-growing global demand for valuable metals, as well as reducing environmental risks. The present study aimed to simultaneously recover both valuable and hazardous metal(loid)s from two sulfidic mine waste samples (waste rock (NC_01) and tailings (NC_02)) from the Neves Corvo mine, Portugal, using a novel acidophilic consortium dominated by iron-oxidizing Leptospirillum genus and Acidiphilium sp. Bioleaching results showed that over 70% of the total Zn, Co, In, As, and Cd contents of NC_01 and NC_02 were leached within 21 days, while 55%–62% Mn was leached. Copper behaved in a refractory manner, as only 33% and 21% Cu were leached from NC_01 and NC_02, respectively. X-ray diffraction (XRD) and Scanning electron microscope-based automated image analyses (SEM/MLA-GXMAP) of the bioleached residues revealed an almost complete absence of residual pyrite in NC_01 and a reduction of pyrite in NC-02, as well as the formation of secondary minerals, especially jarosite. In most cases, the biogenic jarosite co-precipitated some of the leached elements again, e.g., Cu and Pb. In conclusion, a synchronized method for bioleaching valuable and hazardous metal(loid)s was developed using a novel acidophilic consortium, thereby demonstrating the potential for the generation of economic value and environmental risk reduction for sulfidic mine waste samples.

硫化矿山废物通常含有大量有价值和有害的金属（类物质），这可能会带来环境风险，但也可能为资源回收提供机会。通过满足全球对有价值金属不断增长的需求，并降低环境风险，矿山废物的再加工可以提供经济和环境效益。本研究旨在使用一种以铁为主的新型嗜酸联合体，同时从来自葡萄牙内维斯科尔沃矿的两个硫化矿山废物样品（废石 (NC_01) 和尾矿 (NC_02)）中回收有价值和有害金属（类物质） -氧化钩端螺旋菌属和嗜酸菌属. 生物浸出结果表明，NC_01 和 NC_02 的 Zn、Co、In、As 和 Cd 总含量的 70% 以上在 21 天内被浸出，而 55%~62% 的 Mn 被浸出。铜以难熔的方式表现，因为分别从 NC_01 和 NC_02 中浸出 33% 和 21% 的铜。生物浸出残留物的 X 射线衍射 (XRD) 和基于扫描电子显微镜的自动图像分析 (SEM/MLA-GXMAP) 显示，NC_01 中几乎完全没有残留黄铁矿，而 NC-02 中黄铁矿减少，以及次生矿物的形成，尤其是黄钾铁矾。在大多数情况下，生物成因黄钾铁矾再次共沉淀了一些浸出的元素，例如铜和铅。总之，使用一种新的嗜酸联合体开发了一种用于生物浸出有价值和有害金属（类物质）的同步方法，