To establish economically viable production of cyanobacterial bioethanol and commercially valuable bioproducts, mass cultivation is required; however, the exorbitant cost of nutrients is a significant impediment. Currently, potatoes are used extensively in food-processing industries worldwide. These industries produce large quantities of potato peel wastes (PPW), often discarded in the exposed environment, causing ecological problems. As a supplement in the growth medium for cultivation of the marine Synechococcus elongatus BDU 10144, PPW was explored as an inexpensive source of nutrients. Different concentrations of PPW were added to the novel seawater-based medium as a mixotrophic nutritional supplement after physical pretreatment, and biomass and carbohydrate yields were examined for test cyanobacterium. At alkaline pH, PPW supplementation was found to be a promising stimulant for growth and total carbohydrate accumulation, with a marked decline in the cultivation period, doubling carbohydrate synthesis, and significantly increasing bioethanol and co-products production. This study thus demonstrated that 10% PPW supplementation augmented the carbohydrate pool by ~ 2.2-fold and bioethanol yield by ~ 2.3-fold with bioethanol conversion by > 40%. Moreover, the test cyanobacterium was recognized as a prolific producer of high-valued exopolysaccharides (EPS) and mycosporine-like amino acids (MAAs), which were validated using analytical methods like ultraviolet-spectroscopy and high-performance liquid chromatography. Conclusively, PPW could be exploited as a cost-effective, natural, and green nutrient supplement for cyanobacterial mass cultivation, which could aid in devising a sequential strategy for the integrated production of MAAs, EPS, and bioethanol under the “waste-to-wealth” approach.

Graphical abstract

中文翻译：

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一种协同的“变废为宝”的蓝藻生物精炼方法，通过评估马铃薯皮的价值来培养海洋细长聚球藻

为了建立经济可行的蓝藻生物乙醇生产和具有商业价值的生物产品，需要大规模培养；然而，营养物质的高昂成本是一个重大障碍。目前，马铃薯广泛用于全球食品加工行业。这些行业产生大量的马铃薯皮废料（PPW），通常被丢弃在暴露的环境中，造成生态问题。作为培养海洋细长聚球藻的生长培养基的补充物BDU 10144，PPW 被探索为廉价的营养来源。物理预处理后，将不同浓度的 PPW 作为混合营养补充剂添加到新型海水基培养基中，并检查生物量和碳水化合物产量以用于测试蓝藻。在碱性 pH 值下，PPW 补充剂被发现是一种有前途的生长和总碳水化合物积累的刺激剂，其培养期显着下降，碳水化合物合成加倍，并显着增加生物乙醇和副产品的产量。因此，该研究表明，添加 10% 的 PPW 可使碳水化合物库增加约 2.2 倍，生物乙醇产量增加约 2.3 倍，生物乙醇转化率 > 40%。而且，测试蓝藻被认为是高价值胞外多糖 (EPS) 和类菌孢菌素氨基酸 (MAA) 的多产生产者，这些生产者使用紫外光谱和高效液相色谱等分析方法进行了验证。总之，PPW 可以作为一种具有成本效益、天然和绿色的营养补充剂，用于蓝藻大规模培养，这有助于在“废物变富”的情况下制定 MAA、EPS 和生物乙醇综合生产的顺序策略“ 方法。

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