Surface enhanced raman scattering (SERS) technique has been widely implemented for the detection/quantification of numerous compounds. The development of reusable SERS substrates through regeneration is a constant concern of scientists in the field, related to the sustainability of the method. Cold atmospheric plasma (CAP) is an advantageous green method well-known for its effectiveness towards the successful degradation of organic molecules and materials’ activation/modification. In the present study, we explored for the first time nanosecond pulsed dielectric barrier discharge (NSP-DBD) plasma as a rapid, energy efficient method for SERS solid substrates regeneration, implemented either directly in the gas or in the liquid phase as well as through immersion of the substrates in plasma activated water (PAW). We investigated the critical cold plasma factors (e.g. feeding gas, plasma treatment and retention time) in order to propose the most cost-effective alternative and shed light on the underlying regeneration mechanisms. The different SERS analysis case scenarios (analyte's class , concentration andcross check) were considered, in order to simulate real SERS measurements conditions/requirements. In practical terms, such an approach will contribute to a significant reduction of the detection costs, revealing the NSP-DBD process as a flexible, fast, green, effective and low-cost solution towards the SERS substrates regeneration.

表面增强拉曼散射 (SERS) 技术已广泛应用于多种化合物的检测/定量。通过再生开发可重复使用的 SERS 基板是该领域科学家一直关注的问题，与该方法的可持续性有关。冷大气等离子体(CAP) 是一种有利的绿色方法，以其对成功降解有机分子和材料活化/改性的有效性而闻名。在本研究中，我们首次探索了纳秒脉冲介质阻挡放电 (NSP-DBD) 等离子体作为一种快速、节能的 SERS 固体基板再生方法，可直接在气相或液相中以及通过将基板浸入等离子活化水 (PAW) 中。我们调查了关键冷等离子体因素（例如进料气体、等离子体处理和保留时间），以提出最具成本效益的替代方案并阐明潜在的再生机制。为了模拟真实的 SERS 测量条件/要求，考虑了不同的 SERS 分析案例场景（分析物的类别、浓度和交叉检查）。实际上，这种方法将有助于显着降低检测成本，揭示 NSP-DBD 工艺是一种灵活、快速、绿色、有效和低成本的 SERS 基板再生解决方案。