Due to the outstanding properties, magnetic particles have been widely used as magnetic carriers and adsorbents in a diverse range of fields, such as biomedical diagnostics, food safety, and environmental monitoring, etc. Sorting different magnetic particles from each other based on size simultaneously can be very useful in multiple targets separation. To the best of our knowledge, this research firstly proposes the arc comb structure microfluidic (ACSM) chip for multi-magnetic particles separation based on size by magnetophoresis. And the parameters affecting particle trajectory and separation efficiencies, such as flow velocity, velocity ratio of the two inlets, the remanent flux density of the magnets, and dynamic viscosity of the buffer solution, were investigated by numerical simulations in this study. Particle magnetophoresis spectrum width $\Theta$ and general separation ratio $P_{\text{G}}$ are put forward to characterize the particles separation efficiency. Under the recommended simulation condition, six kinds of magnetic beads were separated with a general separation ratio $P_{\text{G}}$ of 0.83 using the newly designed chip. And the separation ratio $p_{\text{i}}$ of the 0.5, 1.8, 2.5, 3, 3.5 and 4.5 µm magnetic particle is 100.00%, 98.18%, 66.36%, 97.25%, 97.27% and 96.36%, respectively. This numerical simulation study provides a theoretical basis for multiple magnetic particles’ separation before experimental trials and also facilitates the utilization and development of microfluidic chips in the future.

由于其优异的性能，磁性颗粒已被广泛用作磁性载体和吸附剂，广泛应用于生物医学诊断、食品安全和环境监测等领域。同时根据大小对不同磁性颗粒进行分类可以在多目标分离中非常有用。据我们所知，本研究首次提出了弧梳结构微流控（ACSM）芯片，用于基于磁泳尺寸的多磁性颗粒分离。并通过数值模拟研究了影响颗粒轨迹和分离效率的参数，如流速、两个入口的速度比、磁体的剩余磁通密度和缓冲溶液的动态粘度。粒子磁泳谱宽$\theta$和一般的分离比${磷}_{\text{G}}$提出了表征颗粒分离效率的方法。在推荐的模拟条件下，以一般的分离比分离了六种磁珠${磷}_{\text{G}}$0.83 使用新设计的芯片。和分离比$p_{\text{一世}}$0.5、1.8、2.5、3、3.5 和 4.5 µm 磁性粒子的比例分别为 100.00%、98.18%、66.36%、97.25%、97.27% 和 96.36%。该数值模拟研究为实验前多个磁性粒子的分离提供了理论依据，也为未来微流控芯片的应用和发展提供了便利。