Amaryllidaceae plants are well known for producing isoquinoline-type alkaloids which have important biological activities, however the production of these secondary metabolites in plants is very low, it’s necessary to study mechanisms to increase the productivity of them. Our study shows the alkaloids composition on the wild bulbs and in vitro bulbs of four species Chilean Amaryllidaceae. Qualitative and semi-quantitative analysis of alkaloid extracts carried out by GC/MS–MS we found a total of 48 different alkaloids produced in wild plants and in vitro culture; 38 alkaloids were identified. These results suggest that different combinations of auxins and cytokinins can modulate plant biochemical pathways that regulate alkaloid biogenesis and show the high potential that plants of the Amaryllidaceae family have to produce lycorine type alkaloids. This study allowed knowing the content of alkaloids in wild and in vitro bulbs of the studied species. These results enable to establish the culture media with 4.44 µM BAP and 2.70 µM NAA and 2.20 µM BAP and 5.40 µM NAA increased the production of lycorine-type alkaloids for R. advena and R. pratensis respectively, therefore it is able to promote oxidative phenol coupling via ortho-para' and demonstrates how it is possible to direct the production of these molecules through the use of phytohormones in in vitro culture. Finally, we can say that the results confirm that micropropagation is a good tool to produce this type of alkaloids.

石蒜科植物以产生具有重要生物活性的异喹啉类生物碱而闻名，但这些次生代谢产物在植物中的产量很低，有必要研究提高它们的生产力的机制。我们的研究显示了四种智利石蒜科野生鳞茎和体外鳞茎上的生物碱成分。通过 GC/MS-MS 对生物碱提取物进行定性和半定量分析，我们发现在野生植物和体外培养中总共产生了 48 种不同的生物碱；鉴定出 38 种生物碱。这些结果表明，生长素和细胞分裂素的不同组合可以调节调节生物碱生物发生的植物生化途径，并显示石蒜科植物产生石蒜碱型生物碱的高潜力。这项研究允许了解所研究物种的野生和体外鳞茎中生物碱的含量。这些结果能够建立具有 4.44 µM BAP 和 2.70 µM NAA 以及 2.20 µM BAP 和 5.40 µM NAA 的培养基，增加了石蒜碱型生物碱的产量R. advena和R. pratensis，因此它能够通过邻对位促进氧化酚偶联，并展示了如何通过在体外培养中使用植物激素来指导这些分子的产生。最后，我们可以说，结果证实了微繁殖是生产这类生物碱的好工具。