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Nanomaterial-modulated cellular sodium extrusion and vacuolar sequestration for salt tolerance
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2022-09-08 , DOI: 10.1039/d2en00623e Feiran Chen 1 , Liqi Zhu 1 , Junfeng Tang 1 , Chuanxi Wang 1 , Le Yue 1 , Peter Schröder 2 , Zhenyu Wang 1
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2022-09-08 , DOI: 10.1039/d2en00623e Feiran Chen 1 , Liqi Zhu 1 , Junfeng Tang 1 , Chuanxi Wang 1 , Le Yue 1 , Peter Schröder 2 , Zhenyu Wang 1
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Nanomaterials can induce plant tolerance to abiotic environmental stresses, whereas the sensing mechanism and the resulting response at the cellular level need further exploration. Bright Yellow 2 tobacco (Nicotiana tabacum L.) suspension-cultured cells challenged with 100 mM NaCl were exposed to increasing doses (0.05, 0.1, and 0.5 mg L−1) of nano-CeO2 (nCeO2) or nitrogen-doped carbon dots (N-CDs). nCeO2 at 0.1 mg L−1 or N-CDs at 0.5 mg L−1 were optimal for alleviating salt stress and nCeO2 was superior to N-CDs. Notably, the expression of genes (IPUT1, SOS3, SOS2, and SOS1) involved in Na+-GIPC perception-Na+-extrusion was stimulated (5.3-fold) after nCeO2 exposure for 4 h, and NHX1 encoding Na+ vacuolar sequestration was induced by 7.8-fold after N-CDs treatment for 6 h. Subsequently, the net Ca2+ influx and Na+ efflux were strongly promoted by 293.6% and 191.3% after incubation with nCeO2, while no obvious Na+ extrusion but the transient K+ influx (by 3.0-fold) was observed for N-CDs. The downstream metabolic modifications in fatty acids, or amino acids and carbohydrates could favor the adaptation to salt stress. This work reveals the specific nanomaterial-induced early cell perception and signaling cascade, contributing to the nano-based agricultural practices for combating salt stress.
中文翻译：
纳米材料调制的细胞钠挤压和液泡隔离用于耐盐性
纳米材料可以诱导植物对非生物环境胁迫的耐受性，而细胞水平的传感机制和由此产生的反应需要进一步探索。用 100 mM NaCl 攻击的亮黄色 2 烟草 ( Nicotiana tabacum L.) 悬浮培养细胞暴露于增加剂量（0.05、0.1 和 0.5 mg L -1）的纳米 CeO 2 (nCeO 2 ) 或氮掺杂碳点（N-CD）。0.1 mg L -1的nCeO 2或0.5 mg L -1的N-CDs对于减轻盐胁迫是最佳的，并且nCeO 2优于N-CDs。值得注意的是，基因的表达（IPUT1，SOS3，SOS2, 和SOS1 ) 在 nCeO 2暴露 4 小时后刺激 (5.3 倍) 参与 Na + -GIPC 感知-Na + -挤出, 在 N-CDs 处理后NHX1编码 Na +液泡隔离被诱导 7.8 倍6小时。随后，与nCeO 2一起孵育后，净Ca 2+流入和Na +流出强烈促进了293.6％和191.3％，而没有明显的Na +挤出，但有短暂的K +观察到 N-CD 的流入量（3.0 倍）。脂肪酸或氨基酸和碳水化合物的下游代谢修饰可能有利于适应盐胁迫。这项工作揭示了特定的纳米材料诱导的早期细胞感知和信号级联，有助于以纳米为基础的农业实践以对抗盐胁迫。
更新日期：2022-09-08
中文翻译：
纳米材料调制的细胞钠挤压和液泡隔离用于耐盐性
纳米材料可以诱导植物对非生物环境胁迫的耐受性，而细胞水平的传感机制和由此产生的反应需要进一步探索。用 100 mM NaCl 攻击的亮黄色 2 烟草 ( Nicotiana tabacum L.) 悬浮培养细胞暴露于增加剂量（0.05、0.1 和 0.5 mg L -1）的纳米 CeO 2 (nCeO 2 ) 或氮掺杂碳点（N-CD）。0.1 mg L -1的nCeO 2或0.5 mg L -1的N-CDs对于减轻盐胁迫是最佳的，并且nCeO 2优于N-CDs。值得注意的是，基因的表达（IPUT1，SOS3，SOS2, 和SOS1 ) 在 nCeO 2暴露 4 小时后刺激 (5.3 倍) 参与 Na + -GIPC 感知-Na + -挤出, 在 N-CDs 处理后NHX1编码 Na +液泡隔离被诱导 7.8 倍6小时。随后，与nCeO 2一起孵育后，净Ca 2+流入和Na +流出强烈促进了293.6％和191.3％，而没有明显的Na +挤出，但有短暂的K +观察到 N-CD 的流入量（3.0 倍）。脂肪酸或氨基酸和碳水化合物的下游代谢修饰可能有利于适应盐胁迫。这项工作揭示了特定的纳米材料诱导的早期细胞感知和信号级联，有助于以纳米为基础的农业实践以对抗盐胁迫。
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