Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-09-07 , DOI: 10.1016/j.jare.2022.08.017 Cijun Shuai 1 , Feng Yang 2 , Yang Shuai 3 , Shuping Peng 4 , Shijie Chen 5 , Youwen Deng 5 , Pei Feng 2
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Introduction
The aggregation of graphene oxide (GO) is considered as main challenge, although GO possesses excellent mechanical properties which arouses widespread attention as reinforcement for polymers.
Objectives
In this study, silicon dioxide (SiO2) nanoparticles were decorated onto surface of GO nanosheets through in situ growth method for promoting dispersion of GO in poly(l-lactic acid) (PLLA) bone scaffold.
Methods
Hydroxyl and carboxyl functional groups of GO provided sites for SiO2 nucleation, and SiO2 grew with hydrolysis and polycondensation of tetraethyl orthosilicate (TEOS) and finally formed nanoparticles onto surface of GO with covalent bonds. Then, the GO@ SiO2 nanocomposite was blended with PLLA for the fabrication of bone scaffold by selective laser sintering (SLS).
Result
The results indicated that the obtained SiO2 were distributed relatively uniformly on surface of GO under TEOS concentration of 0.10 mol/L (GO@SiO2-10), and the covering of SiO2 on GO could increase interlayer distance of GO nanosheets from 0.799 nm to 0.894 nm, thus reducing van der Waals forces between GO nanosheets and facilitating the dispersion. Tensile and compressive strength of scaffold containing GO@SiO2 hybrids were significantly enhanced, especially for the scaffold containing GO@SiO2-10 hybrids with enhancement of 30.95 % in tensile strength and 66.33 % in compressive strength compared with the scaffold containing GO. Additionally, cell adhesion and fluorescence experiments demonstrated excellent cytocompatibility of the scaffold.
Conclusions
The good dispersion of GO@SiO2 enhances the mechanical properties and cytocompatibility of scaffold, making it a potential candidate for bone tissue engineering applications.
中文翻译：
氧化石墨烯纳米片修饰二氧化硅纳米粒子及其在聚乳酸支架中的应用
介绍
氧化石墨烯 (GO) 的聚集被认为是主要挑战，尽管 GO 具有优异的机械性能，作为聚合物的增强材料引起了广泛关注。
目标
在本研究中，二氧化硅 (SiO 2 ) 纳米粒子通过原位生长方法装饰到 GO 纳米片表面，以促进 GO 在聚乳酸 (PLLA) 骨支架中的分散。
方法
GO的羟基和羧基官能团为SiO 2成核提供位点，SiO 2随着原硅酸四乙酯(TEOS)的水解和缩聚而生长，最终通过共价键在GO表面形成纳米粒子。然后，将 GO@ SiO 2纳米复合材料与 PLLA 混合，通过选择性激光烧结 (SLS) 制造骨支架。
结果
结果表明，在TEOS浓度为0.10 mol/L（GO@SiO 2 -10）时，所得SiO 2在GO表面分布较为均匀（GO@SiO 2 -10），SiO 2覆盖在GO上可以增加GO纳米片的层间距离，从0.799 nm 到 0.894 nm，从而减少 GO 纳米片之间的范德华力并促进分散。含有GO@SiO 2杂化物的支架的拉伸和压缩强度显着增强，尤其是含有GO@SiO 2的支架-10 杂种与含有 GO 的支架相比，拉伸强度提高了 30.95%，抗压强度提高了 66.33%。此外，细胞粘附和荧光实验表明支架具有出色的细胞相容性。
结论
GO@SiO 2良好的分散性增强了支架的力学性能和细胞相容性，使其成为骨组织工程应用的潜在候选者。
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