Journal of Natural Medicines ( IF 2.5 ) Pub Date : 2022-09-06 , DOI: 10.1007/s11418-022-01648-9 Chaeyeong Kim 1 , Yun Hee Jeong 2 , Nayeon Kim 1 , Soo Ho Ryu 1 , Jong-Sup Bae 1
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Jujuboside B (JB) found in the seeds of Zizyphi Spinosi Semen possesses pharmacological functions, such as anti-inflammatory, antiplatelet aggregation, and antianxiety potentials. This study evaluated the effect of JB on liver failure in cecal ligation and puncture (CLP)-induced sepsis. First, we observed histopathological changes in the liver by optical microscopy and the activity of enzymes in serum such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST). We further measured the levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, nitric oxide (NO), and antioxidative parameters in liver homogenate. The expression of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), and glucocorticoid receptor (GR) in the liver was observed by Western blotting. CLP enhanced the migration of inflammatory cells, ALT and AST concentrations, and necrosis, which were reduced by JB. In addition, JB reduced 11β-HSD2 expression and levels of inflammatory mediators (TNF-α, IL-1β, and NO) in the liver, increased GR expression, enhanced endogenous antioxidative capacity. These results further suggest that JB may protect the liver against CLP-induced damage by regulating anti-inflammatory responses, downregulating 11β-HSD2 expression and antioxidation, and up-regulating GR expression.
Graphical abstract
中文翻译：
红枣苷B的保肝作用
酸枣仁中的酸枣苷B (JB)精液具有抗炎、抗血小板聚集和抗焦虑等药理功能。本研究评估了 JB 对盲肠结扎穿孔 (CLP) 引起的败血症肝功能衰竭的影响。首先，我们通过光学显微镜观察了肝脏的组织病理学变化和血清中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）等酶的活性。我们进一步测量了肝匀浆中白细胞介素 (IL)-1β、肿瘤坏死因子 (TNF)-α、一氧化氮 (NO) 和抗氧化参数的水平。Western blotting观察肝脏11β-羟基类固醇脱氢酶1型（11β-HSD1）、11β-羟基类固醇脱氢酶2型（11β-HSD2）和糖皮质激素受体（GR）的表达。CLP增强了炎症细胞的迁移，ALT 和 AST 浓度，以及坏死，这些都被 JB 降低了。此外，JB 降低了肝脏中 11β-HSD2 的表达和炎症介质（TNF-α、IL-1β 和 NO）的水平，增加了 GR 的表达，增强了内源性抗氧化能力。这些结果进一步表明，JB 可能通过调节抗炎反应、下调 11β-HSD2 表达和抗氧化以及上调 GR 表达来保护肝脏免受 CLP 诱导的损伤。
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