Radio frequency electric field (RFEF) is a novel technology for the microbial disinfection of liquids such as water and fruit juices. Here we investigated the development of microbial tolerance against RFEF to assess the long-term suitability of this technology. We applied a constant electric field, outlet temperature and treatment time of 14 kV cm⁻¹, 40 °C and 1.275 ms, respectively, to Escherichia coli inoculated in saline water to observe whether the descendants of the surviving bacteria could develop tolerance against RFEF. Escherichia coli that survived the RFEF treatment were recultivated and treated under similar processing conditions for 15 generations.

中文翻译：

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射频电场处理大肠杆菌耐电性的研究

射频电场 (RFEF) 是一种对水和果汁等液体进行微生物消毒的新技术。在这里，我们调查了微生物对 RFEF 耐受性的发展，以评估该技术的长期适用性。我们对接种在盐水中的大肠杆菌施加恒定电场、出口温度和处理时间分别为 14 kV cm ^-1、40 °C 和 1.275 ms，以观察存活细菌的后代是否对 RFEF 产生耐受性。在 RFEF 处理后存活下来的大肠杆菌在类似的处理条件下被重新培养和处理 15 代。