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Effect of cerium on the production of reactive oxygen species in the root of Arabidopsis thaliana: An in vitro study
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-09-03 , DOI: 10.1002/jemt.24226 Keliang Lyu 1 , Zhiwen Lyu 1 , Xinran Zhang 1 , Donghao Hao 1 , Zhonghuang Yang 1 , Yumeng Liu 1 , Dongwu Liu 1 , Xue Wang 1
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-09-03 , DOI: 10.1002/jemt.24226 Keliang Lyu 1 , Zhiwen Lyu 1 , Xinran Zhang 1 , Donghao Hao 1 , Zhonghuang Yang 1 , Yumeng Liu 1 , Dongwu Liu 1 , Xue Wang 1
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In the current study, the effect of trivalent cerium (Ce3+) on the production of reactive oxygen species (ROS) was investigated in the root of Arabidopsis thaliana by an in vitro study. The roots of A. thaliana were exposed with 0, 1, and 5 μmol/L Ce3+ for 12 h in vitro. It was found that the level of H2O2, O2.−, and ·OH was enhanced by 5 μmol/L Ce3+, but reduced by 1 μmol/L Ce3+. The activities of peroxidase (POD), catalase (CAT), and superoxidase dismutase (SOD) were enhanced by 1 μmol/L Ce3+, but reduced by 5 μmol/L Ce3+. Moreover, we used a laser-scanning confocal microscopy to detect the changes of ROS in the root cells of A. thaliana by using a fluorochrome 2′,7′-dichlorofluorescein diacetate (H2DCF-DA). It showed that the level of ROS was declined in the root cells treated by 1 μmol/L Ce3+, but the oscillation of ROS was found in the root cells treated with 5 μmol/L Ce3+. In addition, REEs affect the uptake of mineral elements, which may be related to the oxidative stress in the cells of roots. In all, the data of our study indicated that the appropriate concentration of Ce3+ exhibited an anti-oxidation property and improved the defense system in the root cells of A. thaliana.
中文翻译：
铈对拟南芥根部活性氧产生的影响：一项体外研究
在当前的研究中，通过体外研究研究了三价铈 (Ce 3+ ) 对拟南芥根部活性氧 (ROS) 产生的影响。拟南芥的根在体外暴露于0、1和5μmol/L Ce 3+ 12小时。结果发现，H 2 O 2、O 2 .-和·OH 的水平增加了 5 μmol/L Ce 3+，但减少了 1 μmol/L Ce 3+。1 μmol/L Ce 3+过氧化物酶（POD）、过氧化氢酶（CAT）和超氧化物酶歧化酶（SOD）活性增强，5 μmol/L Ce 3+降低. 此外，我们使用激光扫描共聚焦显微镜通过使用荧光染料 2',7'-二氯荧光素二乙酸酯 (H 2 DCF-DA)来检测拟南芥根细胞中 ROS 的变化。结果表明，1 μmol/L Ce 3+处理的根细胞中ROS水平下降，而5 μmol/L Ce 3+处理的根细胞中ROS水平出现振荡。此外，稀土元素影响矿质元素的吸收，这可能与根系细胞的氧化应激有关。总之，我们的研究数据表明，适当浓度的Ce 3+表现出抗氧化特性并改善了拟南芥根细胞的防御系统。
更新日期：2022-09-03
中文翻译：
铈对拟南芥根部活性氧产生的影响：一项体外研究
在当前的研究中，通过体外研究研究了三价铈 (Ce 3+ ) 对拟南芥根部活性氧 (ROS) 产生的影响。拟南芥的根在体外暴露于0、1和5μmol/L Ce 3+ 12小时。结果发现，H 2 O 2、O 2 .-和·OH 的水平增加了 5 μmol/L Ce 3+，但减少了 1 μmol/L Ce 3+。1 μmol/L Ce 3+过氧化物酶（POD）、过氧化氢酶（CAT）和超氧化物酶歧化酶（SOD）活性增强，5 μmol/L Ce 3+降低. 此外，我们使用激光扫描共聚焦显微镜通过使用荧光染料 2',7'-二氯荧光素二乙酸酯 (H 2 DCF-DA)来检测拟南芥根细胞中 ROS 的变化。结果表明，1 μmol/L Ce 3+处理的根细胞中ROS水平下降，而5 μmol/L Ce 3+处理的根细胞中ROS水平出现振荡。此外，稀土元素影响矿质元素的吸收，这可能与根系细胞的氧化应激有关。总之，我们的研究数据表明，适当浓度的Ce 3+表现出抗氧化特性并改善了拟南芥根细胞的防御系统。
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