To date, knowledge of internal human exposure to BPA and its analogues (particularly bisphenol S and bisphenol F, etc.) remains limited. In the present study, a method involving dispersive solid-phase extraction and LC/MS was proposed to investigate the contamination levels of 28 precursor bisphenols and 9 major metabolites in serum. The critical variables of preparation method were screened out by Plackett-Burman design and further optimized by central composite design. Left in optimal conditions, a total of 286 samples consisting of 153 males and 133 females were analyzed. The results showed that BPA dominated over all the cases with the highest positive rate (82.2% of all the surveyed people), and totally four metabolites (BPA β-D-glucuronide, BPA monosulfate, BPA bis-(β-D-glucuronide) and BPS monosulfate) were detectable. The occurrence of BPA bis-(β-D-glucuronide) in serum is reported for the first time and its higher positive rate and contamination concentrations suggested that it may be a more important metabolite of BPA than others. Negligible potential risk of health effects to blood donors was observed, since the estimated exposure levels (mean 32.1 ng/kg bw/day, 95th 123.2 ng/kg bw/day) were well below far less than the temporary tolerable reference dose of BPA that recommended by the European Food Safety Authority (4 μg/kg bw/day by). The reference level of BPA for healthy population was determined to be 4.09 μg/L via the percentile method.

迄今为止，关于人体内部接触 BPA 及其类似物（特别是双酚 S 和双酚 F 等）的知识仍然有限。在本研究中，提出了一种涉及分散固相萃取和 LC/MS 的方法来研究血清中 28 种前体双酚和 9 种主要代谢物的污染水平。通过 Plackett-Burman 设计筛选出制备方法的关键变量，并通过中心复合设计进一步优化。在最佳条件下，总共分析了 286 个样本，其中包括 153 名男性和 133 名女性。结果显示，BPA在所有病例中占主导地位，阳性率最高（占所有被调查者的82.2%），共有四种代谢物（BPA β-D-葡糖苷酸、BPA单硫酸盐、BPA双-（β-D-葡糖苷酸）和 BPS 单硫酸盐）是可检测到的。首次报道了血清中 BPA bis-(β-D-glucuronide) 的出现，其较高的阳性率和污染浓度表明它可能是 BPA 的一种比其他代谢物更重要的代谢物。观察到对献血者健康影响的潜在风险可以忽略不计，因为估计的暴露水平（平均 32.1 纳克/公斤体重/天，第 95 位 123.2 纳克/公斤体重/天）远低于 BPA 的临时可耐受参考剂量，即由欧洲食品安全局推荐（4 μg/kg bw/day by）。通过百分位数法确定健康人群 BPA 的参考水平为 4.09 μg/L。观察到对献血者健康影响的潜在风险可以忽略不计，因为估计的暴露水平（平均 32.1 纳克/公斤体重/天，第 95 位 123.2 纳克/公斤体重/天）远低于 BPA 的临时可耐受参考剂量，即由欧洲食品安全局推荐（4 μg/kg bw/day by）。通过百分位数法确定健康人群 BPA 的参考水平为 4.09 μg/L。观察到对献血者健康影响的潜在风险可以忽略不计，因为估计的暴露水平（平均 32.1 纳克/公斤体重/天，第 95 位 123.2 纳克/公斤体重/天）远低于 BPA 的临时可耐受参考剂量，即由欧洲食品安全局推荐（4 μg/kg bw/day by）。通过百分位数法确定健康人群 BPA 的参考水平为 4.09 μg/L。