Since interventions such as caloric restriction or fasting robustly promote lipid catabolism and improve aging-related phenotypical markers, we investigated the direct effect of increased lipid catabolism via overexpression of bmm (brummer, FBgn0036449), the major triglyceride hydrolase in Drosophila, on lifespan and physiological fitness. Comprehensive characterization was carried out using RNA-seq, lipidomics and metabolomics analysis. Global overexpression of bmm strongly promoted numerous markers of physiological fitness, including increased female fecundity, fertility maintenance, preserved locomotion activity, increased mitochondrial biogenesis and oxidative metabolism. Increased bmm robustly upregulated the heat shock protein 70 (Hsp70) family of proteins, which equipped the flies with higher resistance to heat, cold, and ER stress via improved proteostasis. Despite improved physiological fitness, bmm overexpression did not extend lifespan. Taken together, these data show that bmm overexpression has broad beneficial effects on physiological fitness, but these effects did not impact lifespan.

中文翻译：

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全身脂肪分解促进果蝇的生理健康

由于热量限制或禁食等干预措施会强有力地促进脂质分解代谢并改善与衰老相关的表型标志物，因此我们研究了果蝇中主要甘油三酯水解酶 bmm（brummer，FBgn0036449）的过度表达增加脂质分解代谢对寿命和生理的直接影响。健康。使用RNA-seq、脂质组学和代谢组学分析进行综合表征。bmm的整体过度表达强烈促进了许多生理健康标志，包括增加女性生育力、维持生育能力、保留运动活性、增加线粒体生物发生和氧化代谢。bmm的增加显着上调了热休克蛋白 70 (Hsp70) 家族蛋白，通过改善蛋白质稳态，使果蝇对热、冷和 ER 应激具有更高的抵抗力。尽管生理健康有所改善，bmm过度表达并没有延长寿命。总而言之，这些数据表明bmm过度表达对生理健康具有广泛的有益影响，但这些影响不会影响寿命。