ABSTRACT

Kidney samples from 300 bat cadavers from the Czech and Slovak Republics were tested for Leptospira DNA using PCR and sequencing of three genes (lipL32, flab, and 16S ribosomal RNA). Overall detection rate was 4.7% and two bat species (Myotis myotis and Nyctalus noctula) were PCR-positive for at least one gene. Detected Leptospira sequences were similar to L. interrogans and L. borgpetersenii, and included a potentially novel species related to L. weilii.

中文翻译：

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捷克和斯洛伐克共和国蝙蝠尸体中钩端螺旋体的检测

摘要

使用 PCR 和三个基因（lipL32、flab和 16S 核糖体 RNA）的测序，对来自捷克和斯洛伐克共和国的 300 具蝙蝠尸体的肾脏样本进行了钩端螺旋体DNA 检测。总检出率为 4.7%，两种蝙蝠（Myotis myotis和Nyctalus noctula）至少有一个基因 PCR 呈阳性。检测到的钩端螺旋体序列与问号L.和L. borgpetersenii 相似，并且包括与L. weilii相关的潜在新物种。