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Single-cell RNA-seq reveals fate determination control of an individual fibre cell initiation in cotton (Gossypium hirsutum)
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-08-29 , DOI: 10.1111/pbi.13918 Yuan Qin 1 , Mengling Sun 1 , Weiwen Li 1 , Mingqi Xu 1 , Lei Shao 1 , Yuqi Liu 1 , Guannan Zhao 1 , Zhenping Liu 1 , Zhongping Xu 1 , Jiaqi You 1 , Zhengxiu Ye 1 , Jiawen Xu 1 , Xiyan Yang 1 , Maojun Wang 1 , Keith Lindsey 2 , Xianlong Zhang 1 , Lili Tu 1
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2022-08-29 , DOI: 10.1111/pbi.13918 Yuan Qin 1 , Mengling Sun 1 , Weiwen Li 1 , Mingqi Xu 1 , Lei Shao 1 , Yuqi Liu 1 , Guannan Zhao 1 , Zhenping Liu 1 , Zhongping Xu 1 , Jiaqi You 1 , Zhengxiu Ye 1 , Jiawen Xu 1 , Xiyan Yang 1 , Maojun Wang 1 , Keith Lindsey 2 , Xianlong Zhang 1 , Lili Tu 1
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Cotton fibre is a unicellular seed trichome, and lint fibre initials per seed as a factor determines fibre yield. However, the mechanisms controlling fibre initiation from ovule epidermis are not understood well enough. Here, with single-cell RNA sequencing (scRNA-seq), a total of 14 535 cells were identified from cotton ovule outer integument of Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis and the day of anthesis). Three major cell types, fibre, non-fibre epidermis and outer pigment layer were identified and then verified by RNA in situ hybridization. A comparative analysis on scRNA-seq data between Xu142 and its fibreless mutant Xu142 fl further confirmed fibre cluster definition. The developmental trajectory of fibre cell was reconstructed, and fibre cell was identified differentiated at 1 day before anthesis. Gene regulatory networks at four stages revealed the spatiotemporal pattern of core transcription factors, and MYB25-like and HOX3 were demonstrated played key roles as commanders in fibre differentiation and tip-biased diffuse growth respectively. A model for early development of a single fibre cell was proposed here, which sheds light on further deciphering mechanism of plant trichome and the improvement of cotton fibre yield.
中文翻译：
单细胞 RNA-seq 揭示了棉花（陆地棉）中单个纤维细胞起始的命运决定控制
棉花纤维是单细胞种子毛状体，每粒种子的皮棉纤维首字母作为决定纤维产量的一个因素。然而，控制纤维从胚珠表皮开始的机制还不是很清楚。在此，通过单细胞 RNA 测序（scRNA-seq），在四个发育阶段（开花前 1.5、1、0.5 天和开花当天）从 Xu142_LF 品系的棉花胚珠外珠被中鉴定出总共 14 535 个细胞。鉴定了三种主要细胞类型、纤维、非纤维表皮和外部色素层，然后通过 RNA原位杂交进行验证。Xu142与其无纤维突变体Xu142 fl的scRNA-seq数据对比分析进一步证实了纤维簇的定义。重建了纤维细胞的发育轨迹，并在开花前1天鉴定出纤维细胞分化。四个阶段的基因调控网络揭示了核心转录因子的时空模式，并证明 MYB25 样和HOX3分别在纤维分化和尖端偏向扩散生长中作为指挥官发挥了关键作用。此处提出了单个纤维细胞的早期发育模型，揭示了植物毛状体的进一步破译机制和棉花纤维产量的提高。
更新日期：2022-08-29
中文翻译：
单细胞 RNA-seq 揭示了棉花（陆地棉）中单个纤维细胞起始的命运决定控制
棉花纤维是单细胞种子毛状体，每粒种子的皮棉纤维首字母作为决定纤维产量的一个因素。然而，控制纤维从胚珠表皮开始的机制还不是很清楚。在此，通过单细胞 RNA 测序（scRNA-seq），在四个发育阶段（开花前 1.5、1、0.5 天和开花当天）从 Xu142_LF 品系的棉花胚珠外珠被中鉴定出总共 14 535 个细胞。鉴定了三种主要细胞类型、纤维、非纤维表皮和外部色素层，然后通过 RNA原位杂交进行验证。Xu142与其无纤维突变体Xu142 fl的scRNA-seq数据对比分析进一步证实了纤维簇的定义。重建了纤维细胞的发育轨迹，并在开花前1天鉴定出纤维细胞分化。四个阶段的基因调控网络揭示了核心转录因子的时空模式，并证明 MYB25 样和HOX3分别在纤维分化和尖端偏向扩散生长中作为指挥官发挥了关键作用。此处提出了单个纤维细胞的早期发育模型，揭示了植物毛状体的进一步破译机制和棉花纤维产量的提高。
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