Toward a Home Test for COVID-19 Diagnosis: DNA Machine for Amplification-Free SARS-CoV-2 Detection in Clinical Samples,ChemMedChem

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Toward a Home Test for COVID-19 Diagnosis: DNA Machine for Amplification-Free SARS-CoV-2 Detection in Clinical Samples
ChemMedChem ( IF 3.6 ) Pub Date : 2022-08-28 , DOI: 10.1002/cmdc.202200382
Ahmed A El-Deeb ₁ , Sofia S Zablotskaya ₁ , Maria S Rubel ₁ , Moustapha A Y Nour ₁ , Liubov I Kozlovskaya ₂ , Anna A Shtro ₃ , Andrey B Komissarov ₃ , Dmitry M Kolpashchikov _{1,

4,

5}

Affiliation

Nucleic acid-based detection of RNA viruses requires an annealing procedure to obtain RNA/probe or RNA/primer complexes for unwinding stable structures of folded viral RNA. We designed a protein-enzyme-free nano-construction, termed a four-armed DNA machine (4DNM), that requires neither an amplification stage nor a high-temperature annealing step for SARS-CoV-2 detection. This strategy can be used for detecting long natural RNA inpoint-of-care or home diagnostic tests.

中文翻译：

实现 COVID-19 诊断的家庭测试：用于临床样本中无扩增 SARS-CoV-2 检测的 DNA 机器

基于核酸的 RNA 病毒检测需要退火程序以获得 RNA/探针或 RNA/引物复合物，以解开折叠病毒 RNA 的稳定结构。我们设计了一种无蛋白质酶的纳米结构，称为四臂 DNA 机器 (4DNM)，它既不需要扩增阶段，也不需要高温退火步骤来检测 SARS-CoV-2。该策略可用于在护理点或家庭诊断测试中检测长天然 RNA。

更新日期：2022-08-28

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