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Mahuang Decoction Attenuates Airway Inflammation and Remodeling in Asthma via Suppression of the SP1/FGFR3/PI3K/AKT Axis
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2022-08-26 , DOI: 10.2147/dddt.s351264 Lina Wei 1 , Xulei Gou 2 , Baoning Su 3 , Haiqiong Han 4 , Tingting Guo 5 , Liang Liu 5 , Lei Wang 5 , Lina Zhang 3 , Weibin Chen 6
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2022-08-26 , DOI: 10.2147/dddt.s351264 Lina Wei 1 , Xulei Gou 2 , Baoning Su 3 , Haiqiong Han 4 , Tingting Guo 5 , Liang Liu 5 , Lei Wang 5 , Lina Zhang 3 , Weibin Chen 6
Affiliation
Background/Purpose: Mahuang decoction (MHD) is a classic famous traditional Chinese medicine and has various pharmacological effects, including anti-inflammation and anti-asthma. In this study, we aimed to investigate the potential protective effect of MHD against asthma and elucidated the underlying mechanism.
Materials and Methods: A mouse model of asthma was induced by ovalbumin (OVA) treatment, and then treated with MHD to evaluate its effect on the asthma. Gain- or loss-of-function approaches were performed in SP1 and FGFR3 to study their roles in asthma via measurement of airway inflammation, airway remodeling and airway smooth muscle cell (ASMC) proliferation-related factors.
Results: MHD reduced airway inflammation and remodeling. Additionally, MHD contributed to diminished expression of SP1, which was shown to repress airway inflammation and remodeling. Furthermore, SP1 bound to the FGFR3 promoter, resulting in the FGFR3 transcription promotion and ASMC proliferation. Conversely, FGFR3 knockdown abolished airway inflammation and remodeling, the mechanism of which was related to suppression of the PI3K/AKT signaling pathway. Meanwhile, MHD hindered airway inflammation and remodeling following asthma by suppressing the SP1/FGFR3/PI3K/AKT axis.
Conclusion: Taken together, MHD may retard airway inflammation and remodeling by suppressing the SP1/FGFR3/PI3K/AKT axis, which contributes to an extensive understanding of asthma and may provide novel therapeutic options for this disease.
Keywords: Mahuang decoction, asthma, SP1, FGFR3, PI3K/AKT signaling pathway, airway inflammation, airway remodeling
中文翻译：
麻黄汤通过抑制 SP1/FGFR3/PI3K/AKT 轴减轻哮喘气道炎症和重塑
背景/目的：麻黄汤（MHD）是经典名药，具有抗炎、平喘等多种药理作用。在这项研究中，我们旨在研究 MHD 对哮喘的潜在保护作用并阐明其潜在机制。
材料与方法：用卵清蛋白（OVA）处理诱导哮喘小鼠模型，然后用MHD处理以评估其对哮喘的影响。通过测量气道炎症、气道重塑和气道平滑肌细胞 (ASMC) 增殖相关因子，在 SP1 和 FGFR3 中进行了获得或丧失功能的方法，以研究它们在哮喘中的作用。
结果：MHD 减少了气道炎症和重塑。此外，MHD 有助于减少 SP1 的表达，这被证明可以抑制气道炎症和重塑。此外，SP1 与 FGFR3 启动子结合，导致 FGFR3 转录促进和 ASMC 增殖。相反，FGFR3 敲低消除了气道炎症和重塑，其机制与抑制 PI3K/AKT 信号通路有关。同时，MHD 通过抑制 SP1/FGFR3/PI3K/AKT 轴来阻碍哮喘后的气道炎症和重塑。
结论：综上所述，MHD 可能通过抑制 SP1/FGFR3/PI3K/AKT 轴来延缓气道炎症和重塑，这有助于广泛了解哮喘，并可能为该疾病提供新的治疗选择。
关键词：麻黄汤，哮喘，SP1，FGFR3，PI3K/AKT信号通路，气道炎症，气道重塑
更新日期：2022-08-25
Materials and Methods: A mouse model of asthma was induced by ovalbumin (OVA) treatment, and then treated with MHD to evaluate its effect on the asthma. Gain- or loss-of-function approaches were performed in SP1 and FGFR3 to study their roles in asthma via measurement of airway inflammation, airway remodeling and airway smooth muscle cell (ASMC) proliferation-related factors.
Results: MHD reduced airway inflammation and remodeling. Additionally, MHD contributed to diminished expression of SP1, which was shown to repress airway inflammation and remodeling. Furthermore, SP1 bound to the FGFR3 promoter, resulting in the FGFR3 transcription promotion and ASMC proliferation. Conversely, FGFR3 knockdown abolished airway inflammation and remodeling, the mechanism of which was related to suppression of the PI3K/AKT signaling pathway. Meanwhile, MHD hindered airway inflammation and remodeling following asthma by suppressing the SP1/FGFR3/PI3K/AKT axis.
Conclusion: Taken together, MHD may retard airway inflammation and remodeling by suppressing the SP1/FGFR3/PI3K/AKT axis, which contributes to an extensive understanding of asthma and may provide novel therapeutic options for this disease.
Keywords: Mahuang decoction, asthma, SP1, FGFR3, PI3K/AKT signaling pathway, airway inflammation, airway remodeling
中文翻译：
麻黄汤通过抑制 SP1/FGFR3/PI3K/AKT 轴减轻哮喘气道炎症和重塑
背景/目的：麻黄汤（MHD）是经典名药，具有抗炎、平喘等多种药理作用。在这项研究中，我们旨在研究 MHD 对哮喘的潜在保护作用并阐明其潜在机制。
材料与方法：用卵清蛋白（OVA）处理诱导哮喘小鼠模型，然后用MHD处理以评估其对哮喘的影响。通过测量气道炎症、气道重塑和气道平滑肌细胞 (ASMC) 增殖相关因子，在 SP1 和 FGFR3 中进行了获得或丧失功能的方法，以研究它们在哮喘中的作用。
结果：MHD 减少了气道炎症和重塑。此外，MHD 有助于减少 SP1 的表达，这被证明可以抑制气道炎症和重塑。此外，SP1 与 FGFR3 启动子结合，导致 FGFR3 转录促进和 ASMC 增殖。相反，FGFR3 敲低消除了气道炎症和重塑，其机制与抑制 PI3K/AKT 信号通路有关。同时，MHD 通过抑制 SP1/FGFR3/PI3K/AKT 轴来阻碍哮喘后的气道炎症和重塑。
结论：综上所述，MHD 可能通过抑制 SP1/FGFR3/PI3K/AKT 轴来延缓气道炎症和重塑，这有助于广泛了解哮喘，并可能为该疾病提供新的治疗选择。
关键词：麻黄汤，哮喘，SP1，FGFR3，PI3K/AKT信号通路，气道炎症，气道重塑
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