Abstract

Background: In recent years, a new type of adipose tissue (beige adipose tissue) has been mentioned, unlike white adipose tissue (WAT) and brown adipose tissue (BAT). Beige cells are capable of thermogenesis like BAT. In response to various agents, beige cells can develop within WAT through a process called “browning.” Therefore, the prevention of obesity and related diseases by providing WAT browning with new potential agents has been extensively studied in recent years. Taurine has many physiological functions in the body and has beneficial effects on obesity and related metabolic disorders. For this reason, we aimed to investigate whether taurine supplementation has effects on browning of WAT and attenuating obesity. Methods: Thirty-two male C57BL/6 mice were used for the study. Mice were divided into 4 groups as control, control + taurine, high fat diet (HFD) and HFD + taurine, and fed for 20 weeks. Taurine was given in drinking water (5%). Epididymal WAT samples were obtained from mice and RNA was extracted from these tissues. Expression levels of FLCN, mTOR, TFE3, PGC-1α, PGC1-1β, AMPK, S6K and UCP1 genes were measured by real-time PCR. Results: Taurine supplementation reduced HFD-induced obesity. No UCP1 expression was detected in any of the groups studied. Any of the gene expressions were not significantly different between HFD and HFD + taurine groups. Reduced PGC-1α and PGC-1β expressions were observed in both HFD and HFD + taurine groups. Conclusions: Taurine reduced the obesity in HFD fed mice, but had no effect on browning of epididymal WAT in this study.

摘要

背景：近年来，一种新型的脂肪组织（米色脂肪组织）被提及，不同于白色脂肪组织（WAT）和棕色脂肪组织（BAT）。米色细胞能够像 BAT 一样产热。为了响应各种试剂，米色细胞可以通过称为“褐变”的过程在 WAT 内发育。因此，近年来广泛研究了通过为 WAT 褐变提供新的潜在药物来预防肥胖和相关疾病。牛磺酸在体内具有多种生理功能，对肥胖和相关代谢紊乱有有益作用。出于这个原因，我们旨在研究补充牛磺酸是否对 WAT 褐变和减轻肥胖有影响。方法：三十二只雄性 C57BL/6 小鼠用于该研究。小鼠分为对照组、对照组+牛磺酸、高脂饮食（HFD）和HFD+牛磺酸4组，喂养20周。在饮用水中加入牛磺酸 (5%)。从小鼠获得附睾 WAT 样本，并从这些组织中提取 RNA。通过实时 PCR 测量 FLCN、mTOR、TFE3、PGC-1α、PGC1-1β、AMPK、S6K 和 UCP1 基因的表达水平。结果：牛磺酸补充剂减少了 HFD 引起的肥胖。在所研究的任何组中均未检测到 UCP1 表达。HFD 和 HFD + 牛磺酸组之间的任何基因表达都没有显着差异。在 HFD 和 HFD + 牛磺酸组中均观察到 PGC-1α 和 PGC-1β 表达减少。结论：在这项研究中，牛磺酸减少了 HFD 喂养小鼠的肥胖，但对附睾 WAT 的褐变没有影响。