The reproductive toxicities over generations are essential to assess the long-term impacts environmental fluoroquinolone antibiotics (FQs). In the present study, the multi-generational effects of ofloxacin (OFL) and norfloxacin (NOR) on reproduction were studied on Caenorhabditis elegans from 9 successive generations (F1-F9). Results showed that OFL showed no effects in F1, stimulation in F2 to F4, and inhibition F5 to F9. The effects of NOR also showed oscillation between stimulation and inhibition across generations. Further biochemical analysis demonstrated that the reproductive toxicities of OFL and NOR were more closely connected with total cholesterol (TCHO), progesterone (P) and testosterone (T), than major sperm protein (MSP) and vitellogenin (Vn). Moreover, OFL and NOR also showed significant trans-generational reproductive toxicities in T4 and T4′, the great-grand-daughter of F1 and F9. Differences between T4 and T4′ and between OFL and NOR, indicated influences of multi-generational exposure and urged more exploration on different mechanisms between FQs.

几代人的生殖毒性对于评估环境氟喹诺酮类抗生素 (FQ) 的长期影响至关重要。在本研究中，研究了氧氟沙星（OFL）和诺氟沙星（NOR）对秀丽隐杆线虫生殖的多代效应。连续 9 代 (F1-F9)。结果表明，OFL对F1无影响，对F2至F4有刺激作用，对F5至F9有抑制作用。NOR 的影响也显示出刺激和抑制之间的跨代振荡。进一步的生化分析表明，与主要精子蛋白 (MSP) 和卵黄素 (Vn) 相比，OFL 和 NOR 的生殖毒性与总胆固醇 (TCHO)、孕酮 (P) 和睾酮 (T) 的关系更为密切。此外，OFL 和 NOR 在 F1 和 F9 的曾孙女 T4 和 T4' 中也显示出显着的跨代生殖毒性。T4和T4'之间以及OFL和NOR之间的差异表明了多代暴露的影响，并敦促对FQ之间的不同机制进行更多探索。