The restoration of bone defects caused by osteoporosis remains a challenge for surgeons. Strontium ranelate has been applied in preventative treatment approaches due to the biological functions of the trace element strontium (Sr). In this study, we aimed to fabricate bioactive scaffolds through Sr incorporation based on our previously developed modified amino-functional mesoporous bioactive glass (MBG) and to systematically investigate the bioactivity of the resulting scaffold in vitro and in vivo in an osteoporotic rat model. The results suggested that Sr-incorporated amino-functional MBG scaffolds possessed favorable biocompatibility. Moreover, with the incorporation of Sr, osteogenic and angiogenic capacities were upregulated in vitro. The in vivo results showed that the Sr-incorporated amino-functional MBG scaffolds achieved better bone regeneration and vessel formation. Furthermore, bioinformatics analysis indicated that the Sr-incorporated amino-functional MBG scaffolds could reduce reactive oxygen species levels in bone marrow mesenchymal stem cells in the osteoporotic model by activating the cAMP/PKA signaling pathway, thus playing an anti-osteoporosis role while promoting osteogenesis. This study demonstrated the feasibility of incorporating trace elements into scaffolds and provided new insights into biomaterial design for facilitating bone regeneration in the treatment of osteoporosis.

由骨质疏松症引起的骨缺损的修复仍然是外科医生面临的挑战。由于微量元素锶 (Sr) 的生物学功能，雷尼酸锶已被应用于预防性治疗方法。在本研究中，我们旨在基于我们之前开发的改性氨基功能介孔生物活性玻璃 (MBG) 通过 Sr 掺入制造生物活性支架，并系统地研究所得支架在骨质疏松大鼠模型中的体外和体内生物活性。结果表明，掺有 Sr 的氨基功能性 MBG 支架具有良好的生物相容性。此外，随着 Sr 的掺入，成骨和血管生成能力在体外被上调。体内结果表明，掺有 Sr 的氨基功能性 MBG 支架实现了更好的骨再生和血管形成。此外，生物信息学分析表明，掺Sr的氨基功能MBG支架可通过激活cAMP/PKA信号通路降低骨质疏松模型中骨髓间充质干细胞中的活性氧水平，从而在促进成骨的同时发挥抗骨质疏松作用。 . 该研究证明了将微量元素纳入支架的可行性，并为促进骨质疏松症治疗中骨再生的生物材料设计提供了新的见解。生物信息学分析表明，掺Sr氨基功能MBG支架可通过激活cAMP/PKA信号通路降低骨质疏松模型骨髓间充质干细胞中的活性氧水平，从而在促进成骨的同时发挥抗骨质疏松作用。该研究证明了将微量元素纳入支架的可行性，并为促进骨质疏松症治疗中骨再生的生物材料设计提供了新的见解。生物信息学分析表明，掺Sr氨基功能MBG支架可通过激活cAMP/PKA信号通路降低骨质疏松模型骨髓间充质干细胞中的活性氧水平，从而在促进成骨的同时发挥抗骨质疏松作用。该研究证明了将微量元素纳入支架的可行性，并为促进骨质疏松症治疗中骨再生的生物材料设计提供了新的见解。