Aim The use of alcohol affects the central nervous system and plays important roles in various neurological disorders through neurotoxicity resulting from blood–brain barrier (BBB) permeability. The BBB is regulated by tight junction proteins interacting closely with endothelial cells. This study evaluated the serum levels of proteins and spectrin degradation products associated with BBB damage in patients with alcohol use disorder. Methods This preliminary case–control study was conducted with 30 healthy volunteers and 26 alcohol use disorder patients. The serum levels of spectrin breakdown product 145 (SBDP145), spectrin breakdown product 150 (SBDP150), ubiquitin carboxy-terminal hydrolase L1 (UCHL1), ubiquitin ligase cullin-3 (ULC), occludin and claudin were measured with enzyme-linked immunosorbent assay. Results There was no significant difference between the levels of SBDP145, SBDP150, UCHL1, ULC, occludin and claudin before and after treatment in patients with alcohol use disorder. SBDP150 levels were significantly lower in patients than controls (P < 0.001). The area under the curve was 0.841 (0.733–0.949) with the 95% confidence interval for SPDP150. Conclusion A decrease of the serum SBDP150 levels appears to be associated with alcohol use disorder. Future studies might clarify whether diminished serum SBDP150 levels are associated with BBB damage in patients with alcohol use disorder.

中文翻译：

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酒精使用障碍患者治疗前后血脑屏障损伤标志物调查的初步研究

目的酒精的使用会影响中枢神经系统，并通过血脑屏障 (BBB) 通透性引起的神经毒性在各种神经系统疾病中发挥重要作用。BBB 由与内皮细胞密切相互作用的紧密连接蛋白调节。本研究评估了酒精使用障碍患者与 BBB 损伤相关的蛋白质和血影蛋白降解产物的血清水平。方法 这项初步的病例对照研究是在 30 名健康志愿者和 26 名酒精使用障碍患者中进行的。酶联免疫吸附法测定血影蛋白分解产物145（SBDP145）、血影蛋白分解产物150（SBDP150）、泛素羧基末端水解酶L1（UCHL1）、泛素连接酶cullin-3（ULC）、occludin和claudin的血清水平. 结果酒精使用障碍患者治疗前后SBDP145、SBDP150、UCHL1、ULC、occludin、claudin水平差异无统计学意义。患者的 SBDP150 水平显着低于对照组 (P < 0.001)。SPDP150 的曲线下面积为 0.841 (0.733–0.949)，置信区间为 95%。结论 血清 SBDP150 水平的降低似乎与酒精使用障碍有关。未来的研究可能会阐明血清 SBDP150 水平降低是否与酒精使用障碍患者的 BBB 损伤有关。SPDP150 的曲线下面积为 0.841 (0.733–0.949)，置信区间为 95%。结论 血清 SBDP150 水平的降低似乎与酒精使用障碍有关。未来的研究可能会阐明血清 SBDP150 水平降低是否与酒精使用障碍患者的 BBB 损伤有关。SPDP150 的曲线下面积为 0.841 (0.733–0.949)，置信区间为 95%。结论 血清 SBDP150 水平的降低似乎与酒精使用障碍有关。未来的研究可能会阐明血清 SBDP150 水平降低是否与酒精使用障碍患者的 BBB 损伤有关。