The incorporation of new information into the hippocampal network is likely to be constrained by its innate architecture and internally generated activity patterns. However, the origin, organization and consequences of such patterns remain poorly understood. In the present study we show that hippocampal network dynamics are affected by sequential neurogenesis. We birthdated CA1 pyramidal neurons with in utero electroporation over 4 embryonic days, encompassing the peak of hippocampal neurogenesis, and compared their functional features in freely moving adult mice. Neurons of the same birthdate displayed distinct connectivity, coactivity across brain states and assembly dynamics. Same-birthdate neurons exhibited overlapping spatial representations, which were maintained across different environments. Overall, the wiring and functional features of CA1 pyramidal neurons reflected a combination of birthdate and the rate of neurogenesis. These observations demonstrate that sequential neurogenesis during embryonic development shapes the preconfigured forms of adult network dynamics.

将新信息整合到海马网络中可能会受到其先天架构和内部生成的活动模式的限制。然而，人们对这种模式的起源、组织和后果仍然知之甚少。在本研究中，我们表明海马网络动力学受顺序神经发生的影响。我们在胚胎 4 天内诞生了子宫内电穿孔的 CA1 锥体神经元，包括海马神经发生的高峰，并比较了它们在自由移动的成年小鼠中的功能特征。相同出生日期的神经元表现出不同的连接性、跨大脑状态的共性和组装动力学。同生日期神经元表现出重叠的空间表示，这些表示在不同的环境中保持不变。总体而言，CA1 锥体神经元的布线和功能特征反映了出生日期和神经发生速率的组合。这些观察结果表明，胚胎发育过程中的连续神经发生塑造了成体网络动力学的预配置形式。