Adipose-derived stem cells (ADSCs) have promising applications in tissue regeneration. Currently, there are only a few ADSC products that have been approved for clinical use. The clinical application of ADSCs still faces many challenges. Here, we review emerging strategies to improve the therapeutic efficacy of ADSCs in tissue regeneration. First, a great quantity of cells is often needed for the stem cell therapies, which requires the advanced cell expansion technologies. In addition cell-derived products are also required for the development of 'cell-free' therapies to overcome the drawbacks of cell-based therapies. Second, it is necessary to strengthen the regenerative functions of ADSCs, including viability, differentiation and paracrine ability, for the tissue repair and regeneration required for different physiological and pathophysiological conditions. Third, poor delivery efficiency also restricts the therapeutic effect of ADSCs. Effective methods to improve cell delivery include alleviating harsh microenvironments, enhancing targeting ability and prolonging cell retention. Moreover, we also point out some critical issues about the sources, effectiveness and safety of ADSCs. With these advanced strategies to improve the therapeutic efficacy of ADSCs, ADSC-based treatment holds great promise for clinical applications in tissue regeneration.

中文翻译：

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改善脂肪干细胞以促进组织再生的策略。

脂肪干细胞（ADSCs）在组织再生中具有广阔的应用前景。目前，只有少数ADSC产品获批临床使用。ADSCs的临床应用仍面临诸多挑战。在这里，我们回顾了提高 ADSC 在组织再生中的治疗效果的新兴策略。首先，干细胞疗法往往需要大量的细胞，这需要先进的细胞扩增技术。此外，开发“无细胞”疗法也需要细胞衍生产品，以克服基于细胞的疗法的缺点。其次，要加强ADSCs的再生功能，包括活力、分化和旁分泌能力，用于不同生理和病理生理条件下所需的组织修复和再生。第三，递送效率差也限制了ADSCs的治疗效果。改善细胞递送的有效方法包括缓解恶劣的微环境、增强靶向能力和延长细胞保留时间。此外，我们还指出了有关 ADSCs 的来源、有效性和安全性的一些关键问题。通过这些先进的策略来提高 ADSC 的治疗效果，基于 ADSC 的治疗在组织再生的临床应用中具有很大的前景。此外，我们还指出了有关 ADSCs 的来源、有效性和安全性的一些关键问题。通过这些先进的策略来提高 ADSC 的治疗效果，基于 ADSC 的治疗在组织再生的临床应用中具有很大的前景。此外，我们还指出了有关 ADSCs 的来源、有效性和安全性的一些关键问题。通过这些先进的策略来提高 ADSC 的治疗效果，基于 ADSC 的治疗在组织再生的临床应用中具有很大的前景。