Mangroves, collection of plants growing in coastal intertidal zones, are rich source of unique bioactive phytochemicals present in their bark, roots, leaves and fruits. They show anti-diabetic, anti-cancer, anti-hypertensive, anti-malarial, anti-bacterial, anti-inflammatory and anti-oxidant properties. In this study, a bioassay-guided isolation and characterization approach was taken to study the anti-bacterial compound from Sonnerartia apetala Buch. Ham leaf. Petroleum ether, acetone and water extracts were prepared and tested against eight human pathogenic bacteria. Water and acetone extracts have shown the inhibition of bacteria but the acetone extract was carried forward for further study. The compound was isolated using preparative column chromatography method. The isolated compound was studied for anti-bacterial activity using TLC-bioautography. The bioactive compound was isolated and tested against standard antibiotics and showed positive results against E. coli and B.subtilis. The isolated pure compound was studied for detection of elements and melting point. Further chacterization was done using UV-Vis spectroscopy and FT-IR spectroscopy indicating absorption in the UV spectrum and presence of aromatic and aliphatic groups. This study validates the importance of Sonneratia apetala leaves as an important resource in traditional medicine for anti-bacterial remedies aimed at human pathogenic diseases.

中文翻译：

---

无瓣海桑中抗菌化合物的生物测定引导分离和表征。火腿叶，来自印度马哈拉施特拉邦海岸

红树林是生长在沿海潮间带的植物群，其树皮、根、叶和果实中含有丰富的独特生物活性植物化学物质。它们具有抗糖尿病、抗癌、抗高血压、抗疟疾、抗菌、抗炎和抗氧化的特性。在这项研究中，采用生物测定引导的分离和表征方法来研究无瓣海桑中的抗菌化合物。火腿叶。制备了石油醚、丙酮和水提取物，并针对八种人类致病菌进行了测试。水和丙酮提取物显示出对细菌的抑制作用，但丙酮提取物被用于进一步研究。使用制备柱色谱法分离该化合物。使用 TLC-bioautography 研究了分离的化合物的抗菌活性。生物活性化合物被分离并针对标准抗生素进行测试，并显示出针对大肠杆菌和枯草杆菌的阳性结果。分离出的纯化合物用于检测元素和熔点。使用 UV-Vis 光谱和 FT-IR 光谱进行进一步表征，表明在 UV 光谱中的吸收以及芳香族和脂肪族基团的存在。本研究证实了无瓣海桑叶作为针对人类致病性疾病的抗菌疗法的传统医学重要资源的重要性。分离出的纯化合物用于检测元素和熔点。使用 UV-Vis 光谱和 FT-IR 光谱进行进一步表征，表明在 UV 光谱中的吸收以及芳香族和脂肪族基团的存在。本研究证实了无瓣海桑叶作为针对人类致病性疾病的抗菌疗法的传统医学重要资源的重要性。分离出的纯化合物用于检测元素和熔点。使用 UV-Vis 光谱和 FT-IR 光谱进行进一步表征，表明在 UV 光谱中的吸收以及芳香族和脂肪族基团的存在。本研究证实了无瓣海桑叶作为针对人类致病性疾病的抗菌疗法的传统医学重要资源的重要性。