Microplastic pollution has attracted growing attention due to its prevalent and persistent exposure to general population through the food chain, but few reports have focused on the toxicological prevention of polystyrene (PS). Using the wild-type and mutant strains, this study explored the impacts of PS and cyanidin-3-O-glucoside (C3G) on stress tolerance and lifespan of Caenorhabditis elegans (C. elegans). In N2 nematodes, PS exposure initiated the oxidative stress and subsequent lifespan reduction, while these adverse impacts could be positively improved by C3G treatment. Considering the pivotal role of DAF-16 pathway in stress tolerance and lifespan regulation, the expression of the daf-16 gene and its downstream antioxidant genes (clt-2, hsp-16.1, sod-3, sod-5) were examined, and found to be significantly enhanced by C3G. Since the sod-3 gene was up-regulated the most fold by C3G, the activity of SOD enzyme that encoded by the sod-3 was examined, and could be obviously enhanced upon C3G treatment. This explained the improved oxidative stress and delayed oxidation-associated aging after C3G intervention. Nevertheless, these positive effects of C3G were weakened in daf-16(-) mutant strain (with deleted DAF-16 gene), for which the beneficial effects of C3G in promoting stress resistance and lifespan extension were inhibited. These findings suggested that the DAF-16 gene and its downstream antioxidant genes, have participated in C3G’s regulations on redox balance and lifespan that impacted by nano-polystyrene particles. This study highlighted the link between dietary components and environmentally driven disturbance.

由于微塑料污染通过食物链普遍且持续地暴露于普通人群，因此引起了越来越多的关注，但很少有报道关注聚苯乙烯 (PS) 的毒理学预防。本研究利用野生型和突变株，探讨了 PS 和 cyanidin-3-O-glucoside (C3G) 对秀丽隐杆线虫( C. elegans ) 的胁迫耐受性和寿命的影响。在 N2 线虫中，PS 暴露引发了氧化应激和随后的寿命缩短，而这些不利影响可以通过 C3G 处理得到积极改善。考虑到 DAF-16 通路在应激耐受和寿命调控中的关键作用，daf-16基因及其下游抗氧化基因 ( clt-2, hsp-16.1 , sod-3 , sod-5 ) 进行了检查，发现 C3G 显着增强。由于sod-3基因被C3G上调最多，因此检测了sod-3编码的SOD酶活性，经C3G处理后可明显增强。这解释了 C3G 干预后改善的氧化应激和延迟的氧化相关衰老。然而，C3G 的这些积极作用在daf-16中被削弱了(-) 突变菌株（缺失 DAF-16 基因），C3G 在促进抗逆性和延长寿命方面的有益作用受到抑制。这些发现表明，DAF-16基因及其下游抗氧化基因参与了C3G对受纳米聚苯乙烯颗粒影响的氧化还原平衡和寿命的调节。这项研究强调了饮食成分与环境驱动干扰之间的联系。