A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H,Chemical Communications

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A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H
Chemical Communications ( IF 4.3 ) Pub Date : 2022-08-18 , DOI: 10.1039/d2cc02411j
Holly A Reeve ₁ , Jake Nicholson ₁ , Farieha Altaf ₁ , Thomas H Lonsdale ₁ , Janina Preissler ₂ , Lars Lauterbach _{2,

3} , Oliver Lenz ₂ , Silke Leimkühler ₄ , Frank Hollmann ₅ , Caroline E Paul ₅ , Kylie A Vincent ₁

Affiliation

We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H₂. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

中文翻译：

一种氢驱动的生物催化方法回收 NAD(P)H 的合成类似物

我们展示了一种合成烟酰胺辅因子类似物的回收系统，该系统使用周转数 >1000 的可溶性氢化酶用于通过 H ₂还原辅因子类似物。将该系统与烯还原酶偶联，我们展示了N-乙基马来酰亚胺到N-乙基琥珀酰亚胺的定量转化。生物催化剂系统在 7 小时后保持 >50% 的活性。

更新日期：2022-08-18

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