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Characterisation of Sr2+ mobility in osteoporotic rat bone marrow by cryo-ToF-SIMS and cryo-OrbiSIMS
Analyst ( IF 3.6 ) Pub Date : 2022-08-18 , DOI: 10.1039/d2an00913g
Christine Kern 1 , Reem Jamous 2 , Thaqif El Khassawna 2 , Marcus Rohnke 1
Affiliation  

Strontium (Sr2+) ions are an effective therapeutic agent for the healing of osteoporotic bone fractures and are therefore used, for example, in form of strontium-modified bone cements. In order to reduce animal testing in further implant materials development in the future, a simulation of the Sr2+ release and transport in bone would be helpful. For such a simulation, knowledge of the experimental parameters for Sr2+ mobility in different compartments of bone (mineralised bone, bone marrow) is essential. In a previous study, we developed an experimental protocol for transport studies in bovine bone marrow by time-of-flight secondary ion mass spectrometry (ToF-SIMS). In the current proof-of-concept study, we investigated Sr2+ diffusion for the first time in bone marrow of rat bone sections. Additionally, orbitrap secondary ion mass spectrometry (OrbiSIMS) was applied for unambiguous signal identification of lipids and fatty acid species in rat bone marrow. Detailed 2D and 3D mass spectrometric imaging analyses, depth profiling as well as OrbiSIMS spectrometric analysis revealed faster Sr2+ diffusion in rat bone marrow areas with low intensity of lipid and fatty acid signals than in areas with higher lipid/fatty acid content. These results could be confirmed by histological staining and additional analysis of Sr2+ diffusion into pure fat sections.

中文翻译:

通过cryo-ToF-SIMS和cryo-OrbiSIMS表征骨质疏松大鼠骨髓中Sr2+的迁移率

锶(Sr 2+ ) 离子是治疗骨质疏松性骨折的有效治疗剂,因此例如以锶改性骨水泥的形式使用。为了在未来进一步的植入材料开发中减少动物试验,模拟 Sr 2+在骨骼中的释放和运输将是有帮助的。对于这样的模拟,了解不同骨区(矿化骨、骨髓)中Sr 2+迁移率的实验参数是必不可少的。在之前的一项研究中,我们通过飞行时间二次离子质谱 (ToF-SIMS) 开发了一种用于牛骨髓运输研究的实验方案。在当前的概念验证研究中,我们研究了 Sr 2+首次在大鼠骨切片的骨髓中扩散。此外,将轨道阱二次离子质谱 (OrbiSIMS) 应用于大鼠骨髓中脂质和脂肪酸种类的明确信号识别。详细的 2D 和 3D 质谱成像分析、深度剖析以及 OrbiSIMS 光谱分析表明,在脂质和脂肪酸信号强度较低的大鼠骨髓区域, Sr 2+扩散比在脂质/脂肪酸含量较高的区域更快。这些结果可以通过组织学染色和额外分析 Sr 2+扩散到纯脂肪切片中得到证实。
更新日期:2022-08-18
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