Shell microelectrode arrays (MEAs) for brain organoids,Science Advances

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Shell microelectrode arrays (MEAs) for brain organoids
Science Advances ( IF 11.7 ) Pub Date : 2022-08-17 , DOI: 10.1126/sciadv.abq5031
Qi Huang ₁ , Bohao Tang ₂ , July Carolina Romero ₃ , Yuqian Yang ₁ , Saifeldeen Khalil Elsayed ₄ , Gayatri Pahapale ₁ , Tien-Jung Lee ₁ , Itzy E Morales Pantoja ₃ , Fang Han ₅ , Cynthia Berlinicke ₆ , Terry Xiang ₁ , Mallory Solazzo ₁ , Thomas Hartung _{3,

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8,

9} , Zhao Qin ₄ , Brian S Caffo ₂ , Lena Smirnova _{3,

8} , David H Gracias _{1,

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Affiliation

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biostatistics, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21287, USA.
Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health and Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY 13244, USA.
Department of Statistics, University of Washington, Seattle, WA 98195, USA.
Department of Ophthalmology, Johns Hopkins University School of Medicine, Wilmer Eye Institute, Baltimore, MD 21287, USA.
CAAT-Europe, University of Konstanz, 78464 Konstanz, Germany.
Environmental Metrology & Policy Program, Georgetown University, Washington, DC, 20057, USA.
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA.
Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Laboratory for Computational Sensing and Robotics (LCSR), Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer–coated metal electrodes. Tunable folding of the minicaps’ polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-μm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.

中文翻译：

用于脑类器官的壳微电极阵列 (MEA)

脑类器官是模仿大脑某些三维（3D）细胞结构和功能的重要模型。多电极阵列（MEA）能够记录和刺激生电细胞的活动，为研究大脑类器官提供了显着的潜力。然而，最初为单层培养设计的传统 MEA 提供的记录接触面积有限，仅限于 3D 类器官的底部。受脑电图帽形状的启发，我们开发了用于类器官的微型晶圆集成 MEA 帽。光学透明的外壳由带有导电聚合物涂层金属电极的自折叠聚合物小叶组成。由力学模拟引导的微型聚合物小叶的可调谐折叠使得能够对不同尺寸的类器官进行多功能记录，并且我们验证了对 400 至 600 μm 大小的类器官进行电生理学记录长达 4 周并响应谷氨酸刺激的可行性。我们的研究表明，3D 壳 MEA 为高信噪比和 3D 时空脑类器官记录提供了巨大潜力。

更新日期：2022-08-17

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