Vertebral discitis-osteomyelitis is an infection of the spine that involves the intervertebral disc and the adjacent vertebral body but may also extend into the paraspinal and epidural soft tissues. If blood cultures and other culture data fail to identify a causative microorganism, percutaneous sampling is indicated to help guide targeted antimicrobial therapy. Despite limited supporting evidence, withholding antimicrobial therapy for up to 2 weeks is recommended to maximize microbiological yield, although literature supporting this recommendation is limited. During the procedure, technical factors that may improve yield include targeting of paraspinal fluid collections or soft tissue abnormalities for sampling, acquiring multiple core samples if possible, and use of larger gauge needles when available. Repeat sampling may be indicated if initial percutaneous biopsy is negative but should be performed no sooner than 72 h after the initial percutaneous biopsy to ensure adequate time for culture results to return.

椎间盘炎-骨髓炎是一种脊柱感染，累及椎间盘和邻近椎体，但也可能延伸到椎旁和硬膜外软组织。如果血培养和其他培养数据无法识别致病微生物，则需要进行经皮取样以帮助指导靶向抗菌治疗。尽管支持证据有限，但建议停止抗菌治疗长达两周，以最大限度地提高微生物产量，尽管支持这一建议的文献有限。在手术过程中，可能提高产量的技术因素包括针对椎旁液体收集或软组织异常进行采样，如果可能的话获取多个核心样本，以及在可用时使用更大规格的针头。如果初次经皮活检呈阴性，则可能需要重复取样，但应在初次经皮活检后 72 小时内进行，以确保有足够的时间返回培养结果。