Cell-Matrix Elastocapillary Interactions Drive Pressure-Based Wetting of Cell Aggregates,Physical Review X

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Cell-Matrix Elastocapillary Interactions Drive Pressure-Based Wetting of Cell Aggregates
Physical Review X ( IF 11.6 ) Pub Date : 2022-08-17 , DOI: 10.1103/physrevx.12.031027
M S Yousafzai _{1,

2} , V Yadav _{1,

2} , S Amiri _{2,

3} , M F Staddon _{4,

5} , Y Errami _{2,

6,

7} , G Jaspard _{1,

2} , S Banerjee ₈ , M Murrell _{1,

2,

9}

Affiliation

The magnitude of mechanical stresses caused by cell surface tension may be comparable to the bulk elasticity of their matrix on cellular length scales, yet how capillary effects influence tissue shape and motion are unknown. In this work, we induce wetting (spreading and migration) of cell aggregates, as models of active droplets onto adhesive substrates of varying elasticity, and correlate the dynamics of wetting to the balance of interfacial tensions. Upon wetting rigid substrates, cell-substrate tension drives outward expansion of the monolayer. By contrast, upon wetting compliant substrates, cell-substrate tension is attenuated and aggregate capillary forces contribute to internal pressures that drive expansion. Thus, we show by experiments, data-driven modeling, and computational simulations that myosin-driven “active elastocapillary” effects enable adaptation of wetting mechanisms to substrate rigidity and introduce a novel, pressure-based mechanism for guiding collective cell motion.

中文翻译：

细胞-基质弹性毛细管相互作用驱动细胞聚集体的基于压力的润湿

由细胞表面张力引起的机械应力的大小可能与其基质在细胞长度尺度上的体积弹性相当，但毛细管效应如何影响组织形状和运动尚不清楚。在这项工作中，我们诱导细胞聚集体的润湿（扩散和迁移），作为不同弹性的粘合基底上的活性液滴的模型，并将润湿动力学与界面张力的平衡相关联。当润湿刚性基质时，细胞-基质张力驱动单层向外扩张。相比之下，在润湿顺应性基质时，细胞-基质张力减弱，并且聚集的毛细管力有助于驱动膨胀的内部压力。因此，我们通过实验、数据驱动建模和计算模拟表明，肌球蛋白驱动的“主动弹性毛细管”效应能够使润湿机制适应基底刚性，并引入一种新颖的、基于压力的机制来引导集体细胞运动。

更新日期：2022-08-17

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