Abstract

Reference intervals are essential for correct interpretation of laboratory test results, supporting clinicians in distinguishing between healthy and sick individuals. The present study aims to establish pediatric reference intervals for hematological parameters based on a large population of healthy schoolchildren. Blood samples were obtained from 1351 children 6–12 years of age participating in the Health-Oriented Pedagogical Project (HOPP). Reference intervals for hematological parameters were estimated by the nonparametric method following the CLSI C28-A3 guidelines. Reference intervals were estimated as 2.5th and 97.5th percentiles with corresponding 90% confidence intervals. While hematocrit and MCV required age and sex partitioning, hemoglobin and erythrocytes were partitioned for age. The remaining parameters, MCH, MCHC, platelets and white blood cell counts did not require partitioning. While red blood cell parameters exhibited an increasing trend with age, there was a slight decrease in leukocytes, lymphocytes, basophils and platelets with age. The remaining parameters were stable across our age span.

摘要

参考区间对于正确解释实验室测试结果至关重要，支持临床医生区分健康个体和患病个体。本研究旨在建立基于大量健康学童的血液学参数的儿科参考区间。血液样本来自 1351 名 6-12 岁参与以健康为导向的教育项目 (HOPP) 的儿童。根据 CLSI C28-A3 指南，通过非参数方法估计血液学参数的参考区间。参考区间估计为第 2.5 和第 97.5 百分位数，相应的置信区间为 90%。虽然血细胞比容和 MCV 需要年龄和性别划分，但血红蛋白和红细胞是根据年龄划分的。其余参数，MCH，MCHC，血小板和白细胞计数不需要分区。虽然红细胞参数随年龄增长呈上升趋势，但白细胞、淋巴细胞、嗜碱性粒细胞和血小板随年龄增长略有下降。其余参数在我们的年龄跨度内保持稳定。